Synthesis of photoreactive pullulan for surface modification
Synthesis of photoreactive pullulan for surface modification
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DOI：
10.1016/j.biomaterials.2004.07.065
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Ito, Y
中科院分区：
文献类型：
--
作者：
Hasuda, H;Kwon, OH;Ito, Y
制备了光反应性普鲁兰多糖，将其光敏化在聚合物或有机表面，并研究了其与蛋白质和一种细胞类型的相互作用。通过与4-叠氮苯甲酸的偶联反应合成了光反应性普鲁兰多糖。在存在或不存在微图案光罩的情况下进行表面改性，该光罩包含具有150微米间隙的100微米透明条纹，从而易于确认固定化。通过微图案化方法，证实了光反应普鲁兰在聚苯乙烯、聚乙烯和硅烷偶联玻璃上的固定化。在未形成图案的表面上测量接触角。虽然原始表面有不同的接触角，但Az-Pullulan固定化表面的接触角在所有表面上都是相同的。这一结果表明，光固定化完全覆盖了Az-Pullulan的表面。将荧光标记的白蛋白应用到微图案化的表面上，研究了蛋白质的吸附：荧光显微镜显示，普鲁兰多糖固定的区域的吸附减少。将来源于小鼠白血病单核细胞的RAW264细胞在微图案化表面培养22h，发现细胞不附着于固定化的普鲁兰多糖区。总之，光反应普鲁兰多糖被共价固定在不同的表面上，并倾向于减少与蛋白质和细胞的相互作用。(C)2004爱思唯尔有限公司。保留所有权利。
Photoreactive pullulan was prepared, the polymer was photommiobilized on polymeric or organic surfaces, and its interactions with a protein and a cell type were investigated. The photoreactive pullulan was synthesized by a coupling reaction with 4-azidobenzonic acid. Surface modification was carried out in the presence or absence of a micropatterned photornask containing 100 mum transparent stripes with 150 mum gaps, making it easy to confirm the immobilization. By the micropatterning method, immobilization of the photoreactive pullulan on polystyrene, polyethylene, and silane-coupled glass was confirmed. Contact angles were measured on the unpatterned surfaces. Although the original surfaces have different contact angles, the contact angle on Az-pullulan-immobilized surface was the same on all surfaces. This result demonstrated that photoirnmobilization completely covered the surface with Az-pullulan. Protein adsorption was investigated using fluorescently labeled albumin applied to the micropatterned surface: fluorescence microscopy demonstrated that adsorption was reduced on the pullulan-immobilized regions. Culture of RAW264 cells, derived from mouse leukemic monocytes, on the micropatterned surface for 22 h showed that cells did not,adhere to the immobilized pullulan regions. In conclusion, photoreactive pullulan was covalently immobilized on various surfaces and tended to reduce interactions with proteins and cells. (C) 2004 Elsevier Ltd. All rights reserved.