The effects of transplacental porcine circovirus type 2 infection on porcine epidemic diarrhoea virus-induced enteritis in preweaning piglets
The effects of transplacental porcine circovirus type 2 infection on porcine epidemic diarrhoea virus-induced enteritis in preweaning piglets
复制标题
DOI：
10.1016/j.tvjl.2005.02.016
复制
发表时间：
2006-05-01
影响因子：
2.2
通讯作者：
Chae, C.
中科院分区：
文献类型：
--
作者：
Jung, K.;Kim, J.;Chae, C.
研究了经胎盘感染猪圆环病毒2型（PCV 2）对猪流行性腹泻病毒（PEDV）诱发仔猪肠炎的影响。将6头妊娠母猪随机分配至感染组（n = 3）或对照组（n = 3）。在预期分娩日期前3周，用6 mL组织培养液鼻内接种3头妊娠母猪，该组织培养液含有1.2 x 10（5）组织培养感染剂量50%（TCID 50）/mL的PCV 2菌株SNUVR 000470。将三头对照妊娠母猪类似地暴露于未感染的细胞培养物上清液。将来自PCV 2感染母猪的30头仔猪随机分配至两组（A和B），每组15头仔猪。将来自未感染母猪的另外30头仔猪随机分配到两组（C和D），每组15头仔猪。A组和C组中的仔猪在三日龄时在第三次传代时经口给予2 mL PEDV毒株SNUVR 971496的病毒原液（1 X 10（6.5）TCID 50/mL）JV 2感染母猪的PEDV感染仔猪（A组）的平均绒毛高度和隐窝深度（VH：CD）比率在接种后36、48和72小时（hpi）与来自PCV 2阴性母猪的PEDV感染仔猪（C组）的平均绒毛高度和隐窝深度（VH：CD）比率显著不同（P < 0.05）。在来自PCV 2感染母猪的PEDV感染仔猪（A组）中，在感染后24 h在空肠组织中检测到的PEDV核酸显著多于来自PCV 2阴性母猪的PEDV感染仔猪（C组）的相同组织（P < 0.05）。此后，在36、48、60和70 hpi时，在来自PCV 2阴性母猪的PEDV感染仔猪（C组）的空肠组织中检测到的PEDV核酸（P < 0.05）显著多于来自PCV 2感染母猪的PEDV感染仔猪（A组）的空肠组织中检测到的PEDV核酸。得出结论，PEDV疾病的临床病程明显受到PCV 2经胎盘感染的影响。(c)2005爱思唯尔有限公司保留所有权利。
The effects of transplacental porcine circovirus type 2 (PCV2) infection on porcine epidemic diarrhoea virus (PEDV)-induced enteritis were examined in neonatal piglets. Six pregnant sows were randomly allocated to an infected (n = 3) or control group (n = 3). Three pregnant sows were inoculated intranasally with 6 mL of tissue culture fluid containing 1.2 x 10(5) tissue culture infective doses 50% (TCID50)/mL of PCV2 strain SNUVR000470 three weeks before the expected farrowing date. Three control pregnant sows were similarly exposed to uninfected cell culture supernatants. Thirty piglets from PCV2-infected sows were randomly assigned to two groups (A and B) of 15 piglets each. Another 30 piglets from noninfected sows were randomly assigned to two groups (C and D) of 15 piglets each. The piglets in groups A and C were dosed orally at three days of age with 2 mL of virus stock (1 X 10(6.5) TCID50/mL) of the PEDV strain, SNUVR971496, at the third passage.The mean villous height and crypt depth (VH:CD) ratio in PEDV-infected piglets from PCV2-infected sows (group A) were significantly different from those of the PEDV-infected piglets from PCV2 negative sows (group C) at 36, 48, and 72 It post-inoculation (hpi) (P < 0.05). In PEDV-infected piglets from PCV2-infected sows (group A), significantly more PEDV nucleic acid was detected in the jejunal tissues (P < 0.05) at 24 hpi than in the same tissues of the PEDV-infected piglets from PCV2 negative sows (group C). Thereafter, at 36, 48, 60, and 70 hpi significantly more PEDV nucleic acid (P < 0.05) was detected in the jejunal tissues of the PEDV-infected piglets from PCV2 negative sows (group C) than those of the PEDV-infected piglets from the PCV2-infected sows (group A). It is concluded that the clinical course of PEDV disease was markedly affected by transplacental infection of PCV2. (c) 2005 Elsevier Ltd. All rights reserved.