Analysis of gene-specific DNA damage and repair using quantitative polymerase chain reaction
Analysis of gene-specific DNA damage and repair using quantitative polymerase chain reaction
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DOI：
10.1006/meth.2000.1054
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发表时间：
2000-10-01
期刊：
影响因子：
4.8
通讯作者：
Van Houten, B
中科院分区：
文献类型：
--
作者：
Ayala-Torres, S;Chen, YM;Van Houten, B
聚合酶链反应（PCR）发现后不久，各个实验室就尝试使用定量PCR（QPCR）来检测特定基因片段中的DNA损伤。促进长PCR的技术的发展增加了检测的灵敏度，从而可以评估DNA损伤剂的生物学相关剂量。QPCR已被用于调查不同的遗传毒物诱导的DNA损伤，并建立许多基因的修复动力学。目前的工作旨在分析暴露于特定DNA损伤剂（如氧化应激）的动物特定基因的损伤和修复。(C)北京大学出版社.
Soon after discovery of the polymerase chain reaction (PCR), various laboratories have attempted to use quantitative PCR (QPCR) to detect DNA damage in specific gene segments. The development of techniques that facilitate long PCR increased the sensitivity of the assay so that biologically relevant doses of DNA-damaging agents could be assessed. QPCR has been used to survey DNA damage induced by different genotoxicants and to establish the repair kinetics of numerous genes. Current work seeks to analyze damage and repair in specific genes from animals exposed to specific DNA-damaging agents such as oxidative stress. (C) 2000 Academic Press.