Connexin36 is essential for transmission of rod-mediated visual signals in the mammalian retina
Connexin36 is essential for transmission of rod-mediated visual signals in the mammalian retina
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DOI：
10.1016/s0896-6273(02)01046-2
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发表时间：
2002-11-14
期刊：
影响因子：
16.2
通讯作者：
Paul, DL
中科院分区：
文献类型：
--
作者：
Deans, MR;Volgyi, B;Paul, DL
为了检查电突触在从视杆光感受器到神经节细胞的信号传输中的功能，我们培育了 connexin36 敲除小鼠。报告基因表达表明 connexin36 存在于多个视网膜神经元中，包括视杆细胞、视锥双极细胞和所有无长突细胞。通过细胞内注射神经生物素证明了相邻 Ails 之间以及 Ails 和 ON 视锥双极之间电突触的破坏。此外，敲除中的细胞外记录揭示了神经节细胞水平上杆介导的中心反应的完全消除。这些数据直接证明电突触对于杆状信号在哺乳动物视网膜上的传播至关重要，并且它们证明了多个杆状通路的存在，每条通路都依赖于电突触。
To examine the functions of electrical synapses in the transmission of signals from rod photoreceptors to ganglion cells, we generated connexin36 knockout mice. Reporter expression indicated that connexin36 was present in multiple retinal neurons including rod photoreceptors, cone bipolar cells, and All amacrine cells. Disruption of electrical synapses between adjacent Ails and between Ails and ON cone bipolars was demonstrated by intracellular injection of Neurobiotin. In addition, extracellular recording in the knockout revealed the complete elimination of rod-mediated, on-center responses at the ganglion cell level. These data represent direct proof that electrical synapses are critical for the propagation of rod signals across the mammalian retina, and they demonstrate the existence of multiple rod pathways, each of which is dependent on electrical synapses.