Laser-stimulated fluorescence in paleontology.

Laser-stimulated fluorescence in paleontology.
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古生物学中的激光激发荧光

DOI:
10.1371/journal.pone.0125923
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发表时间:
2015
期刊:
影响因子:
3.7
通讯作者:
Wang Y
Wang Y
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Kaye TG;Falk AR;Pittman M;Sereno PC;Martin LD;Burnham DA;Gong E;Xu X;Wang Y

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在过去的十年中,使用紫外(UV)光的荧光作为古生物学的工具得到了越来越多的使用。激光激发荧光(LSF)是一种新一代技术,它是一种使在典型紫外线下保持黑暗的古生物标本发出荧光的方法。激光在宏观和微观水平上都能集中非常高的通量率,这导致样品以标准紫外线灯泡无法诱导的方式发出荧光。这里介绍的是五个古生物学案例的历史,说明了在广泛的标本和规模的技术。新的用途,如背光不透明的标本,以揭示细节和检测标本完全掩盖矩阵突出在这些例子中。最近中等功率短波长激光器的成本降低和标准照相滤光片的使用使这项技术被研究人员广泛使用。这项技术有可能使古生物学的多个方面自动化,包括微化石的制备和分类。这是解决古生物学准备瓶颈的一种极具成本效益的方法。
Fluorescence using ultraviolet (UV) light has seen increased use as a tool in paleontology over the last decade. Laser-stimulated fluorescence (LSF) is a next generation technique that is emerging as a way to fluoresce paleontological specimens that remain dark under typical UV. A laser’s ability to concentrate very high flux rates both at the macroscopic and microscopic levels results in specimens fluorescing in ways a standard UV bulb cannot induce. Presented here are five paleontological case histories that illustrate the technique across a broad range of specimens and scales. Novel uses such as back-lighting opaque specimens to reveal detail and detection of specimens completely obscured by matrix are highlighted in these examples. The recent cost reductions in medium-power short wavelength lasers and use of standard photographic filters has now made this technique widely accessible to researchers. This technology has the potential to automate multiple aspects of paleontology, including preparation and sorting of microfossils. This represents a highly cost-effective way to address paleontology's preparatory bottleneck.
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发表时间: 2005-06-07
影响因子: 4.7
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