Analysis of liver and gill miRNAs of Larimichthys crocea against Cryptocryon irritans challenge
Analysis of liver and gill miRNAs of Larimichthys crocea against Cryptocryon irritans challenge
复制标题
大黄鱼肝脏和鳃 miRNA 对抗隐毛虫攻击的分析。
DOI：
10.1016/j.fsi.2016.10.027
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发表时间：
2016-12-01
影响因子：
4.7
通讯作者：
Zheng, Wei-Qiang
中科院分区：
文献类型：
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作者：
Qiao, Ying;Mao, Yong;Zheng, Wei-Qiang
由海洋纤毛虫引起的大黄鱼白斑病阻碍了大黄鱼养殖业的可持续发展。在分子水平上更好地了解寄生虫与寄主的相互作用，将有助于预防L大黄鱼由白斑病引起的大规模死亡。MicroRNAs(MiRNAs)是一类约20-22个核苷酸的小RNA分子，它在转录后调节许多蛋白质编码基因，参与许多生物学过程，特别是在宿主-病原体反应中。本研究利用SolexA高通量测序技术，在L大黄鱼的鳃和肝脏中发现了已知和新的miRNAs。进一步研究这些数据以筛选差异表达的miRNAs，并预测它们的靶基因。肝组织中有103个miRNAs在文库间存在差异表达(p<；0.05)，其中65个和38个是保守的，67个和36个是上调和下调的miRNAs。而在鳃组织中发现了122个差异表达的miRNAs，其中83个和39个是保守的，新的miRNAs有79个和43个是上调和下调的miRNAs。此外，这些差异表达的miRNAs还针对一系列基因，这些基因参与了包括免疫反应在内的许多重要生物学过程。在这里，我们通过深度测序，首次表征了大黄鱼对刺激C菌攻击的响应，这一结果将有助于更好地了解大黄鱼在刺激杆菌攻击下的免疫反应。(C)2016爱思唯尔有限公司。保留所有权利。
The white-spot disease caused by marine ciliate Cryptocryon irritans hindered the sustainable development of large yellow croaker Larimichthys crocea industry. Better understandings about the parasite-host interactions in the molecular level will facilitate the prevention of mass mortality of the L crocea caused by white-spot disease. MicroRNAs (miRNAs) are a class of small RNA molecules about 20-22 nucleotides which post-transcriptionally regulated many protein-coding genes and involved in many biological processes, especially in host-pathogen responses. In this study, we identified known and novel miRNAs in the gill and liver of L crocea challenged by C irritans by high throughput sequencing using Solexa technology. The data were further studied to screen differentially expressed miRNAs, and predict their target genes. The differential expression (p < 0.05) between libraries was observed in 103 miRNAs in liver tissue, among which 65 and 38 were conserved and novel miRNAs, 67 and 36 were up-and down regulated miRNAs. While in gill tissue, 122 significant differentially expressed miRNAs were identified, among which 83 and 39 were conserved and novel miRNAs, 79 and 43 were up-and down-regulated miRNAs. In addition, these differentially expressed miRNAs target a series of genes which involved in many important biological processes including immune response. Here via deep sequencing, we for the first time characterize L. crocea miRNAs in response to C irritans challenge, the results should help for better understandings about the immune response of the L. crocea under C. irritans challenge. (C) 2016 Elsevier Ltd. All rights reserved.