Characterization of a novel insect-specific flavivirus from Brazil: potential for inhibition of infection of arthropod cells with medically important flaviviruses.
Characterization of a novel insect-specific flavivirus from Brazil: potential for inhibition of infection of arthropod cells with medically important flaviviruses.
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DOI：
10.1099/vir.0.068031-0
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Brault AC
中科院分区：
文献类型：
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作者：
Kenney JL;Solberg OD;Langevin SA;Brault AC
在过去的十年中，在全球范围内分离的新描述的昆虫特异性黄病毒的数量激增。我们最近描述了一种新的黄病毒（暂命名为“Nhumirim病毒”; NHUV）的分离，它代表了一个独特的子集，显然是昆虫特异性病毒的一个例子，该病毒在遗传学上与双宿主蚊媒黄病毒有关，尽管似乎仅限于在蚊子细胞中复制。我们的特点是在体外生长潜力，3'非翻译区（UTR）序列的同源性与替代黄病毒，并评估病毒的能力，以抑制复制的代表性库蚊属。在蚊子细胞中传播致病性黄病毒。只有蚊子细胞系被发现支持NHUV复制，进一步加强了这种病毒的昆虫特异性表型。对3' UTR的序列和预测的RNA二级结构的分析表明NHUV与黄热病血清群、日本脑炎血清群内的病毒和蜱传黄病毒进化枝中的病毒最相似。与传统的昆虫特异性黄病毒相比，发现NHUV共享最少的保守序列元件。这表明，尽管这种病毒显然是昆虫特异性的，但它可能与祖先的蚊媒黄病毒不同。共感染实验表明，先前或同时用NHUV感染蚊子细胞会导致西尼罗河病毒（WNV）、圣路易斯脑炎病毒（SLEV）和日本脑炎病毒的病毒产量显着减少。抑制作用对WNV和SLEV最有效，峰值滴度分别降低超过100万倍和10，000倍。
In the past decade there has been an upsurge in the number of newly described insect-specific flaviviruses isolated pan-globally. We recently described the isolation of a novel flavivirus (tentatively designated “Nhumirim virus”; NHUV) that represents an example of a unique subset of apparently insect-specific viruses that phylogenetically affiliate with dual-host mosquito-borne flaviviruses despite appearing to be limited to replication in mosquito cells. We characterized the in vitro growth potential, 3’ untranslated region (UTR) sequence homology with alternative flaviviruses, and evaluated the virus’s capacity to suppress replication of representative Culex spp. vectored pathogenic flaviviruses in mosquito cells. Only mosquito cell lines were found to support NHUV replication, further reinforcing the insect-specific phenotype of this virus. Analysis of the sequence and predicted RNA secondary structures of the 3’ UTR indicate NHUV to be most similar to viruses within the yellow fever serogroup, Japanese encephalitis serogroup, and viruses in the tick-borne flavivirus clade. NHUV was found to share the fewest conserved sequence elements when compared to traditional insect-specific flaviviruses. This suggests that, despite being apparently insect-specific, this virus likely diverged from an ancestral mosquito-borne flavivirus. Co-infection experiments indicated that prior or concurrent infection of mosquito cells with NHUV resulted in significant reduction in viral production of West Nile virus (WNV), St. Louis encephalitis virus (SLEV) and Japanese encephalitis virus. The inhibitory effect was most effective against WNV and SLEV with over a million-fold and 10,000-fold reduction in peak titers, respectively.