Mechanostructure and composition of highly reproducible decellularized liver matrices
Mechanostructure and composition of highly reproducible decellularized liver matrices
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DOI：
10.1016/j.actbio.2013.10.023
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发表时间：
2014-02-01
影响因子：
9.7
通讯作者：
Ahluwalia, A.
中科院分区：
文献类型：
--
作者：
Mattei, G.;Di Patria, V.;Ahluwalia, A.
尽管有关脱细胞支架的论文越来越多，但对于生物组织脱细胞的最佳方法，以尽可能保留基质的微结构和蛋白质含量，目前还没有达成共识。因此，本研究以肝脏为重点，旨在开发一种生产特征良好且可重复的基质的方法，最好地保留天然细胞外基质 (ECM) 的结构和组成。鉴于基质刚度在调节细胞反应中的重要性，还考虑了脱细胞组织的机械性能。测试和分析框架基于在相同的可重复初始状态（即平衡膨胀状态）下对脱细胞和未经处理的样品进行表征。使用生化、组织学、机械和结构分析对脱细胞 ECM (dECM) 进行表征，以确定最佳程序，以确保完全去除细胞，同时保留大部分天然 ECM 结构和组成。利用该方法，以组织或基质的平衡膨胀状态为参考，以一致且可重复的方式获得了具有高度保守的小叶内微结构和蛋白质含量的无菌脱细胞猪ECM。相对于天然组织，观察到肝脏 dECM 的压缩弹性模量显着降低，这表明需要重新检查用于体外工程组织的 ECM 模拟支架的设计参数。 (C) 2013 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Despite the increasing number of papers on decellularized scaffolds, there is little consensus on the optimum method of decellularizing biological tissue such that the micro-architecture and protein content of the matrix are conserved as far as possible. Focusing on the liver, the aim of this study was therefore to develop a method for the production of well-characterized and reproducible matrices that best preserves the structure and composition of the native extra cellular matrix (ECM). Given the importance of matrix stiffness in regulating cell response, the mechanical properties of the decellularized tissue were also considered. The testing and analysis framework is based on the characterization of decellularized and untreated samples in the same reproducible initial state (i.e., the equilibrium swollen state). Decellularized ECM (dECM) were characterized using biochemical, histological, mechanical and structural analyses to identify the best procedure to ensure complete cell removal while preserving most of the native ECM structure and composition. Using this method, sterile decellularized porcine ECM with highly conserved intra-lobular micro-structure and protein content were obtained in a consistent and reproducible manner using the equilibrium swollen state of tissue or matrix as a reference. A significant reduction in the compressive elastic modulus was observed for liver dECM with respect to native tissue, suggesting a re-examination of design parameters for ECM-mimicking scaffolds for engineering tissues in vitro. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.