SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex
SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex
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DOI：
10.1016/s1097-2765(00)80184-7
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发表时间：
1999-07-01
期刊：
影响因子：
16
通讯作者：
Hieter, P
中科院分区：
文献类型：
--
作者：
Kitagawa, K;Skowyra, D;Hieter, P
我们已经确定SGT 1作为skp 1 -4的剂量抑制因子，skp 1 -4是一种导致酵母动粒功能缺陷的突变。Sgt 1 p在体内和体外与Skp 1 p物理结合。SGT 1是一个必需基因，不同的SGT 1条件突变体具有G1或G2 DNA含量。Sgt 1 -3（G2等位基因）突变体的遗传和表型分析支持着丝粒功能的重要作用。Sgt 1 p是通过激活Ctf 13 p组装酵母动粒复合物CBF 3所必需的。Sgt 1 p还与SCF（Skp 1 p/Cdc 53 p/F box protein）泛素连接酶结合。sgt 1 -5（G1等位基因）突变体在体内Sic 1 p周转和体外Cln 1 p泛素化中有缺陷。人SGT 1挽救了一个sgt 1无效突变，表明SGT 1的功能在进化中是保守的。
We have identified SGT1 as a dosage suppressor of skp1-4, a mutation causing defects in yeast kinetochore function. Sgt1p physically associates with Skp1p in vivo and in vitro. SGT1 is an essential gene, and different sgt1 conditional mutants arrest with either a G1 or G2 DNA content. Genetic and phenotypic analyses of sgt1-3 (G2 allele) mutants support an essential role in kinetochore function. Sgt1p is required for assembling the yeast kinetochore complex, CBF3, via activation of Ctf13p. Sgt1p also associates with SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase. sgt1-5 (G1 allele) mutants are defective in Sic1p turnover in vivo and Cln1p ubiquitination in vitro. Human SGT1 rescues an sgt1 null mutation, suggesting that the function of SGT1 is conserved in evolution.