Defining the Protein Complex Proteome of Plant Mitochondria
Defining the Protein Complex Proteome of Plant Mitochondria
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DOI：
10.1104/pp.111.182352
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发表时间：
2011-10-01
期刊：
影响因子：
7.4
通讯作者：
Braun, Hans-Peter
中科院分区：
文献类型：
--
作者：
Klodmann, Jennifer;Senkler, Michael;Braun, Hans-Peter
一个经典的方法，蛋白质分离的二维蓝色原生/十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，串联质谱和最新的计算机技术相结合，以表征的线粒体“蛋白质复合体蛋白质组”的拟南芥（拟南芥）在迄今为止无与伦比的深度。我们进一步开发了新的GelMap软件包来注释和评估二维蓝色原生/十二烷基硫酸钠凝胶。该软件允许（1）根据功能和结构相关性（例如不同蛋白质复合物的亚基）注释蛋白质，（2）将综合蛋白质鉴定列表分配给单个凝胶点，从而（3）选择性显示低丰度的蛋白质复合物。总共有471种不同的蛋白质被质谱鉴定，其中几种形成至少35种不同的线粒体蛋白质复合物的一部分。据我们所知，许多蛋白质复合物首次被描述（例如，包括参与核酸代谢的五肽重复蛋白的复合物）。在我们的数据集中发现更多的蛋白质复合物是通过公共GelMap门户网站www.gelmap.de/arabidopsis_mito向所有人开放的。
A classical approach, protein separation by two-dimensional blue native/sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was combined with tandem mass spectrometry and up-to-date computer technology to characterize the mitochondrial "protein complex proteome" of Arabidopsis (Arabidopsis thaliana) in so far unrivaled depth. We further developed the novel GelMap software package to annotate and evaluate two-dimensional blue native/sodium dodecyl sulfate gels. The software allows (1) annotation of proteins according to functional and structural correlations (e.g. subunits of a distinct protein complex), (2) assignment of comprehensive protein identification lists to individual gel spots, and thereby (3) selective display of protein complexes of low abundance. In total, 471 distinct proteins were identified by mass spectrometry, several of which form part of at least 35 different mitochondrial protein complexes. To our knowledge, numerous protein complexes were described for the first time (e.g. complexes including pentatricopeptide repeat proteins involved in nucleic acid metabolism). Discovery of further protein complexes within our data set is open to everybody via the public GelMap portal at www.gelmap.de/arabidopsis_mito.