Segregation Distortion Through Female Gametophytes in Interspecific Hybrids of Tetraploid Wheat as Revealed by RAPD Analysis
Segregation Distortion Through Female Gametophytes in Interspecific Hybrids of Tetraploid Wheat as Revealed by RAPD Analysis
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RAPD 分析揭示四倍体小麦种间杂种中雌配子体的分离扭曲
DOI：
10.1111/j.1601-5223.1999.00047.x
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发表时间：
2004
期刊：
影响因子：
2.7
通讯作者：
C. Nakamura
中科院分区：
文献类型：
--
作者：
M. Manabe;T. Ino;M. Kasaya;S. Takumi;N. Mori;I. Ohtsuka;C. Nakamura
对外高加索地区特有的5种四倍体小麦进行种间杂交，研究染色体标记分离。膨松小麦（Triticum turgidum）与提尔诺菲伊小麦（T. tirnopheevi）的反向杂交产生了不育的F1s，而硬粒小麦（T. durum）与T的杂交组合产生了3个反向F1s。pericurn， T. durum/T。古结肠菌和T. turgidum/T.；紫堇可育，自受精后产生整倍体F2后代。20个RAPD标记是通过对3个可育的互易F1s基因型的测定而得到的，其中6个RAPD标记是通过D基因组染色体替换系分配到单个染色体上的。f2的分离分析表明，20个核标记中有5个几乎完全或优先从单亲遗传。通过对硬粒绦虫/硬粒绦虫杂交组合BC1后代的分析发现，硬粒绦虫在3A染色体上的一个标记完全通过雌配子传递。persicum。这种通过雌性配子的完全分离扭曲与f15中显著减少的自交结实率相关。在古葡萄球菌与肿胀葡萄球菌的杂交中，还发现了与细胞质相关的紫葡萄球菌标记的优先传播。
Interspecific crosses were made among five tetraploid wheat species including three endemic species in Transcaucasia to study chromosome marker segregation. A reciprocal cross between Triticum turgidum and T. tirnopheevi produced sterile F1s, while three reciprocal F1s obtained from cross combinations of T. durum/T. persicurn, T. durum/T. paleocolchicum and T. turgidum/T. puleocolchicum were fertile and produced euploid F2 progeny after self fertilization. Twenty RAPD markers were is nuclear markers specific to one or two species by determining the genotypes of the three fertile reciprocal F1s, and six were assigned to individual chromosomes using the D genome chromosome substitution lines. Segregation analysis in the F2s showed that five among the 20 nuclear markers were almost completely or preferentially transmitted from single parents. A complete transmission through the female gametes of one T. durum marker on chromosome 3A was shown by the analysis of BC1 progeny from the cross combination of T. durum/T. persicum. This complete segregation distortion through the female gametes was correlated with markedly reduced selfed seed set in the F1s. Preferential transmission of a T. puleocolchicum marker associated with the cytoplasm was also detected in a cross of T. paleocolchicurn x T. turgidum.