Angiopoietins differentially influence in vitro angiogenesis by endothelial cells of different origin
Angiopoietins differentially influence in vitro angiogenesis by endothelial cells of different origin
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DOI：
10.3233/ch-2011-1393
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发表时间：
2011-01-01
影响因子：
2.1
通讯作者：
Bahramsoltani, Mahtab
中科院分区：
文献类型：
--
作者：
De Spiegelaere, Ward;Cornillie, Pieter;Bahramsoltani, Mahtab
血管生成素是血管发育和静止的重要生长因子。它们是多种病理中促或抗血管生成治疗的有希望的靶点，但ANGPT/TIE 2系统的机制复杂且尚未得到充分理解。在本研究中，血管生成素1和血管生成素2的单独和联合作用进行了研究，使用最近开发的体外血管生成模型，允许定量和定性评价的血管生成级联。该细胞培养模型用源自不同血管床的微血管内皮细胞（EC）（即真皮EC和心脏EC）进行。此外，用RT-qPCR分析血管生成素和受体TIE 1和TIE 2的表达。这项研究表明，血管生成素引起了两种内皮细胞培养物的差异反应。血管生成素1和血管生成素2在皮肤EC中引起血管生成级联反应，但在心脏EC中不引起血管生成级联反应。此外，RT-qPCR数据显示这些因子的内源性表达模式存在显著差异，表明内皮细胞的起源可能对其血管生成潜力产生重要影响。
Angiopoietins are important growth factors for vascular development and quiescence. They are promising targets for pro-or anti-angiogenic therapies in diverse pathologies, but the mechanisms of the ANGPT/TIE2 system are complex and not well understood. In the present study, the separate and combined effects of angiopoietin 1 and angiopoietin 2 were studied, using a recently developed in vitro angiogenesis model that allows both a quantitative and qualitative evaluation of the angiogenic cascade. This cell culture model was performed with microvascular endothelial cells (ECs) originating from different vascular beds, i.e. dermal ECs and cardiac ECs. In addition, the expression of the angiopoietins and the receptors, TIE1 and TIE2 was analyzed with RT-qPCR. This study revealed that the angiopoietins provoked a differential response in the two endothelial cultures. Both angiopoietin 1 as well as angiopoietin 2 elicited an angiogenic cascade in the dermal ECs but not in the cardiac ECs. In addition, the RT-qPCR data revealed marked differences in the endogenous expression pattern of these factors, indicating that the origin of endothelial cells might have an important impact on their angiogenic potential.