Submicrometer metallic barcodes
Submicrometer metallic barcodes
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DOI：
10.1126/science.294.5540.137
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发表时间：
2001-10-05
期刊：
影响因子：
56.9
通讯作者：
Natan, MJ
中科院分区：
文献类型：
--
作者：
Nicewarner-Peña, SR;Freeman, RG;Natan, MJ
我们合成了多金属微棒，本质上编码亚微米条纹。通过将金属离子顺序电化学沉积到具有均匀尺寸的孔的模板中，容易地制备复杂的条纹图案。相邻条纹的不同反射率使得能够通过常规的紧密显微镜来识别条纹图案。这种读出机制不干扰使用荧光检测结合到颗粒的亲和捕获的分析物，如DNA和蛋白质生物测定证明。
We synthesized multimetal microrods intrinsically encoded with submicrometer stripes. Complex striping patterns are readily prepared by sequential electrochemical deposition of metal ions into templates with uniformly sized pores. The differential reflectivity of adjacent stripes enables identification of the striping patterns by conventional tight microscopy. This readout mechanism does not interfere with the use of fluorescence for detection of analytes bound to particles by affinity capture, as demonstrated by DNA and protein bioassays.