Truncation of TRIM5 in the Feliformia Explains the Absence of Retroviral Restriction in Cells of the Domestic Cat
Truncation of TRIM5 in the Feliformia Explains the Absence of Retroviral Restriction in Cells of the Domestic Cat
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DOI：
10.1128/jvi.00670-09
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发表时间：
2009-08-15
影响因子：
5.4
通讯作者：
Willett, Brian J.
中科院分区：
文献类型：
--
作者：
McEwan, William A.;Schaller, Torsten;Willett, Brian J.
TRIM5 α在多种哺乳动物物种中介导一种有效的逆转录病毒限制性表型。在这里，我们在猫细胞中发现了一个TRIM5转录物，该转录物具有截断的B30.2衣壳结合域和减弱的限制功能，值得注意的是，该转录物在整个Feliformia中都是保守的。猫TRIM5没有表现出限制性活性，但异位表达对人类TRIM5 α具有显性的负作用。我们的研究结果解释了猫细胞中没有逆转录病毒限制，并表明TRIM5位点的破坏在食肉动物中至少两次独立出现，这与该分类单元中宿主和病原体的进化有关。
TRIM5 alpha mediates a potent retroviral restriction phenotype in diverse mammalian species. Here, we identify a TRIM5 transcript in cat cells with a truncated B30.2 capsid binding domain and ablated restrictive function which, remarkably, is conserved across the Feliformia. Cat TRIM5 displayed no restriction activity, but ectopic expression conferred a dominant negative effect against human TRIM5 alpha. Our findings explain the absence of retroviral restriction in cat cells and suggest that disruption of the TRIM5 locus has arisen independently at least twice in the Carnivora, with implications concerning the evolution of the host and pathogen in this taxon.