Uterine-conceptus interactions and reproductive failure in cattle
Uterine-conceptus interactions and reproductive failure in cattle
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DOI：
10.1016/s0093-691x(01)00645-8
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发表时间：
2001-12-01
期刊：
影响因子：
2.8
通讯作者：
Pru, JK
中科院分区：
文献类型：
--
作者：
Thatcher, WW;Guzeloglu, A;Pru, JK
母体滋养外胚层细胞与子宫内膜上皮细胞之间的对话。对CL维持和胚胎存活至关重要。研究了牛干扰素(IFN)-tau调节牛子宫内膜（BEND）细胞环氧化酶(COX)-2表达和前列腺素F-2 α (PGF(2 α))分泌的信号转导机制。phorbol酯（phorbol 12,13 dibutyrate [PDBu]）刺激蛋白激酶C可通过丝裂原活化蛋白激酶（MAPK）途径激活COX-2基因表达和PGF（2 α）分泌。干扰素-tau减弱PDBu对PGF（2 α）分泌的激活，但这种抑制作用似乎独立于MAPK途径。胚胎IFN-tau通过I型IFN受体作用，激活Janus激酶(JAK)-信号转换器和转录激活因子（STAT）途径，导致干扰素刺激基因的激活或抑制。实验证据表明，IFN-tau对STATs的调控通过减少PGF（2 α）的分泌来调节COX-2的基因表达。本文讨论了母体对抗黄体解药途径的调控，涉及多不饱和脂肪酸二十碳五烯酸（EPA）减少子宫内膜PGF（2 α）分泌的能力，以及黄体酮增加妊娠发育和IFN-tau分泌的能力。(C) 2001年Elsevier Science Inc.
The dialogue between trophectoderm cells of the conceptus and epithelial cells of the endometrium. is critical to CL maintenance and embryo survival. The signal transduction mechanisms by which bovine interferon (IFN)-tau regulates cyclooxygenase (COX)-2 expression and secretion of prostaglandin F-2 alpha (PGF(2 alpha)) in bovine endometrial (BEND) cells is examined. Stimulation of Protein Kinase C with a phorbol ester (phorbol 12, 13 dibutyrate [PDBu]) activates COX-2 gene expression and PGF(2 alpha) secretion via the mitogen-activated protein kinase (MAPK) pathway. Interferon-tau attenuates PDBu activation of PGF(2 alpha), secretion, but this inhibitory effect appears to be independent of the MAPK pathway. Embryonic IFN-tau, acting through a Type I IFN receptor, activates the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway resulting in activation or repression of interferon-stimulated genes. Experimental evidence is provided that IFN-tau regulation of STATs regulates gene expression of COX-2 in a manner that decreases secretion of PGF(2 alpha), Maternal regulation of the antiluteolytic pathway is discussed relative to the ability of the polyunsaturated fatty acid, eicosapentaenoic (EPA), to decrease endometrial secretion of PGF(2 alpha), and progesterone to increase both conceptus development and IFN-tau secretion. (C) 2001 by Elsevier Science Inc.