RELATIONSHIP BETWEEN ABNORMAL SOMITE DEVELOPMENT AND AXIAL SKELETAL DEFECTS IN RATS FOLLOWING HEAT EXPOSURE
RELATIONSHIP BETWEEN ABNORMAL SOMITE DEVELOPMENT AND AXIAL SKELETAL DEFECTS IN RATS FOLLOWING HEAT EXPOSURE
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DOI：
10.1002/tera.1420480309
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发表时间：
1993-09-01
期刊：
影响因子：
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通讯作者：
KIMMEL, CA
中科院分区：
文献类型：
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作者：
CUFF, JM;KIMMEL, GL;KIMMEL, CA
在GD 11评价了妊娠第10天（GD）大鼠胚胎体内热暴露的影响，以确定体内和体外暴露后形态学后遗症之间的关系，以及GD 11检测到的影响与出生后第3天（PND）幼仔中观察到的影响之间的关系。将麻醉大鼠暴露于42 ℃的暖空气培养箱中，直至其直肠温度达到41 ℃或直至直肠温度维持在42-42.5 ℃ 5分钟。与对照组相比，热暴露胚胎的生长参数（包括头长、体节数和蛋白质含量/胚胎）显著降低。这些变化与早期研究（G.L. Kimmel等人，'93）。在形态学终点中，尾神经管、鳃条、前肢和后肢的发育略有延迟。对胚胎的唯一影响，不能解释为一个短暂的延迟，由热诱导的发育是诱导不分节体节。将额外的胚胎在体外暴露于42 ℃ 15-20分钟，并专门检查未分割的体节，在体内或体外暴露于42 ℃的胚胎中观察到47%的体节。这种现象在体节9-20中观察到，即，那些导致颈椎、胸椎和肋骨的骨骼。这些结果与暴露于相同热处理的PND 3幼仔中观察到的轴向骨骼畸形良好相关（C.A. Kimmel等人，'93）。(C)1993 Wiley-Liss，Inc.
The effects of in vivo heat exposure on gestation day (GD) 10 rat embryos were evaluated on GD 11 to determine the relationships between morphological sequelae following in vivo and in vitro exposures and between effects detected on GD 11 and those observed in postnatal day (PND) 3 pups. Anesthetized rats were exposed to 42-degrees-C in a warm air incubator until their rectal temperatures reached 41-degrees-C or until a rectal temperature of 42-42.5-degrees-C had been maintained for 5 minutes. Heat-exposed embryos exhibited a significant decrease in growth parameters including head length, somite number, and protein content/embryo versus controls. These changes correlated well with in vitro effects from an earlier study (G.L. Kimmel et al., '93). Among the morphological endpoints which were slightly delayed in development were the caudal neural tube, branchial bars, forelimb and hindlimb. The only effect on the embryos that could not be explained as a transient delay in development induced by heat was the induction of unsegmented somites. Additional embryos were exposed to 42-degrees-C for 15-20 min in vitro and examined specifically for unsegmented somites, which were observed in 47% of embryos exposed to 42-degrees-C in vivo or in vitro. This phenomenon was observed in somites 9-20, i.e., those that give rise to cervical and thoracic vertebrae and ribs. These results correlated well with the axial skeletal malformations observed in PND 3 pups exposed to the same heat treatment (C.A. Kimmel et al., '93). (C) 1993 Wiley-Liss, Inc.