Nude mice of different strains in parabiosis for prolonged periods of time.
Nude mice of different strains in parabiosis for prolonged periods of time.
复制标题
不同品系的裸鼠长时间共生。
DOI：
10.1159/000163244
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
J. Ledbetter
中科院分区：
文献类型：
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作者：
B. Giovanella;J. T. Hunter;D. W. Gary;J. Stehlin;J. Ledbetter
自本世纪初以来，联体共生已广泛应用于生物学研究。然而，由于排斥现象的存在，其应用受到限制，除非同一近交系的动物融合。将裸基因引入不同品系的小鼠和大鼠中，彻底改变了这种情况。由于这些动物不存在排斥反应，现在可以将属于不同品系并具有不同生物学特性的联体共生个体放入其中。我们已经对 500 多对裸鼠进行了联体共生，它们渴望两种品系——NIH-1 和 NIH-2。 NIH-1 裸鼠是携带 nu 基因的瑞士近交系小鼠，胸腺缺陷，但 B 细胞系统几乎正常。 NIH-2 小鼠是携带 x 连锁免疫缺陷基因 (XID) 和 nu 基因的 CBA 近交系小鼠。因此，这些小鼠胸腺缺陷，并且还具有与循环抗体缺乏相关的 B 细胞缺陷。通过对具有不同免疫能力的动物进行联体共生，我们试图深入了解参与控制免疫反应的调节机制。我们实验室的主要目标之一是通过研究各种治疗药物对移植到裸鼠体内的人类肿瘤的效果，更好地治疗人类癌症。我们之前曾报道过，一些人类肿瘤细胞系皮下接种到成年NIH-1裸鼠时不会生长，但接种到NIH-2小鼠、特权部位或新生NIH-1裸鼠时会生长[1。 2]。我们相信，对仿生小鼠体内人类肿瘤植入物生长的研究将使我们能够成功植入更多、更多种的人类肿瘤。并了解裸鼠体内某些肿瘤不像其他肿瘤那样容易生长的原因。在本文中，我们报告了第一项研究的结果。
Parabiosis has been extensively used in bio logical research since the start of this century. However, it has been limited in its application by the presence of rejection phenomena, ex cept when animals of the same inbred strain are fused. The introduction of the nude gene into different strains of mice and rats has dras tically altered this situation. Due to the ab sence of rejection reactions in these animals, it is now possible to put in parabiosis individuals belonging to different strains and having different biological characteristics. We have parabiosed over 500 pairs of nude mice be longing to two strains-NIH-1 and NIH-2. The NIH-1 nudes are Swiss outbred mice car rying the nu gene and are thymus-deficient but have a nearly normal B cell system. The NIH-2 mice are CBA outbred mice carrying the x-linked immunodeficiency gene (XID) as well as the nu gene. Thus, these mice are thymus-deficient and also have a B-cell defect associated with a deficiency of circulating antibodies. By parabiosing animals with different immunologic capabilities, we seek to gain insight into the regulatory mechanisms involved in the control of the immune re sponse. One of the major goals of our labora tory is to treat human cancer better by study ing the effects of various therapeutic agents on human tumors transplanted in nude mice. We have previously reported that some human tu mor cell lines do not grow when inoculated subcutaneously into adult NIH-1 nude mice but will grow when inoculated into NIH-2 mice, privileged sites, or newborn NIH-1 nude mice [1. 2]. We believe that studies of the growth of human tumor implants in para biosed mice will enable us to successfully im plant more and various kinds of human tu mors. and to understand the reasons why some tumors do not grow as readily as others in the nude mouse. In this paper, we report the results of our first studies.