Copper-mediated cross-linking of S100A4, but not of S100A2, results in proinflammatory effects in melanoma cells
Copper-mediated cross-linking of S100A4, but not of S100A2, results in proinflammatory effects in melanoma cells
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DOI：
10.1016/j.bbrc.2011.08.132
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发表时间：
2011-09-30
影响因子：
3.1
通讯作者：
Pietzsch, Jens
中科院分区：
文献类型：
--
作者：
Haase-Kohn, Cathleen;Wolf, Susann;Pietzsch, Jens
本研究的目的是研究fe -hand钙结合蛋白S100家族的两个成员S100A2和S100A4对铜介导的交联的反应及其生理后果。正如电泳和质谱技术所证明的那样，S100A2和S100A4显示了由于铜介导的半胱氨酸残基氧化而形成的交联。对于S100A4，而不是S100A2，这导致人类A375中NF κ B的激活和tnf - α的分泌增加，并且在更大程度上在rage转染的黑色素瘤细胞中增加。这些数据表明，促氧化肿瘤微环境可增强S100A4的促炎和促转移作用。(C) 2011爱思唯尔公司版权所有。
The aim of this study was to investigate the response to and the physiological consequences of copper-mediated cross-linking of S100A2 and S100A4, two members of the S100 family of EF-hand calcium-binding proteins. As demonstrated by electrophoresis and mass spectrometry techniques S100A2 and S100A4 show formation of cross-links due to copper-mediated oxidation of cysteine residues. For S100A4, but not for S100A2, this results in both increased activation of NF kappa B and secretion of TNF-alpha in human A375 and, to a higher extent, in RAGE-transfected melanoma cells. The data suggest that a prooxidative tumor microenvironment enhances proinflammatory and prometastatic action of S100A4. (C) 2011 Elsevier Inc. All rights reserved.