ISOLATION OF A GENE-CLUSTER FROM XANTHOMONAS-CAMPESTRIS PV VESICATORIA THAT DETERMINES PATHOGENICITY AND THE HYPERSENSITIVE RESPONSE ON PEPPER AND TOMATO
ISOLATION OF A GENE-CLUSTER FROM XANTHOMONAS-CAMPESTRIS PV VESICATORIA THAT DETERMINES PATHOGENICITY AND THE HYPERSENSITIVE RESPONSE ON PEPPER AND TOMATO
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DOI：
10.1094/mpmi-4-081
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发表时间：
1991-01-01
影响因子：
3.5
通讯作者：
STALL, RE
中科院分区：
文献类型：
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作者：
BONAS, U;SCHULTE, R;STALL, RE
野油菜黄单胞菌致病变种的非致病突变体。已经失去在抗性宿主植物和非宿主中诱导过敏反应能力的vesicatoria由来自野生型文库的克隆补充。 对转座子插入突变体的分析确定了X基因组的25-内切酶区域。C. PV. vesicatoria，这是细菌与植物相互作用所必需的。 这一区域的基因被命名为hrp基因。 互补研究表明，hrp区域被组织成至少六个不同的互补组。 Southern杂交结果表明，与hrp簇中的23-脱氢酶区域同源的DNA序列存在于X.野油菜 在某些致病型中，同源性高到足以允许标记基因的交换或非致病突变体的X。C. PV.囊膜序列 通过杂交分析，发现了黄毛壳菌的hrp序列之间的交叉杂交。C. PV.未观察到不同种假单胞菌的基因组DNA。
Nonpathogenic mutants of Xanthomonas campestris pv. vesicatoria that had lost the ability to induce a hypersensitive response in resistant host plants and in nonhosts were complemented by clones from a wild-type library. Analysis of transposon insertion mutants defined a 25-kilobase region of the genome of X. c. pv. vesicatoria, which is necessary for the interaction of the bacterium with the plant. The genes in this region were designated hrp genes. Complementation studies revealed that the hrp region is organized into at least six different complementation groups. Southern blot experiments showed that DNA sequences homologous to a 23-kilobase region from the hrp cluster were present in other pathovars of X. campestris. In some pathovars homology was high enough to permit marker gene exchange or complementation of nonpathogenic mutants by X. c. pv. vesicatoria sequences. Cross-hybridization between the hrp sequences of X. c. pv. vesicatoria and genomic DNA of different species of Pseudomonas was not observed.