Evidence of intra-segmental homologous recombination in influenza A virus.
Evidence of intra-segmental homologous recombination in influenza A virus.
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DOI：
10.1016/j.gene.2011.04.012
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发表时间：
2011-08-01
期刊：
影响因子：
3.5
通讯作者：
Hao W
中科院分区：
文献类型：
--
作者：
Hao W
流感病毒的进化是非常动态的。流感病毒在序列上迅速进化，并经历不同基因片段的频繁重配。同源重组，虽然通常被认为是许多其他生物体中动态基因组进化的重要组成部分，但据信在流感中是罕见的。在这项研究中，256个基因片段从32个甲型流感病毒基因组进行了同源重组，检测到三个重组H1N1毒株，它们很可能是由一个重组事件之间的两个密切评级的父母序列。这些发现表明，流感病毒中的同源重组往往发生在具有高度序列相似性的毒株之间。3株重组菌在不同时间段分离得到，并形成一个分支，表明重组菌可以循环使用。此外，模拟结果表明，当亲本序列具有高序列相似性时，许多重组序列可能无法被现有的重组检测程序检测到。最后，讨论了提高流感病毒重组序列检测准确性的可能途径。
The evolution of influenza viruses is remarkably dynamic. Influenza viruses evolve rapidly in sequence and undergo frequent reassortment of different gene segments. Homologous recombination, although commonly seen as an important component of dynamic genome evolution in many other organisms, is believed to be rare in influenza. In this study, 256 gene segments from 32 influenza A genomes were examined for homologous recombination, three recombinant H1N1 strains were detected and they most likely resulted from one recombination event between two closely rated parental sequences. These findings suggest that homologous recombination in influenza viruses tends to take place between strains sharing high sequence similarity. The three recombinant strains were isolated at different time periods and they form a clade, indicating that recombinant strains could circulate. In addition, the simulation results showed that many recombinant sequences might not be detectable by currently existing recombinant detection programs when the parental sequences are of high sequence similarity. Finally, possible ways were discussed to improve the accuracy of the detection for recombinant sequences in influenza.
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影响因子：
3
作者：
Hao W
通讯作者：
Hao W
影响因子：
3
作者：
Chan CX;Beiko RG;Ragan MA
通讯作者：
Ragan MA
DOI：
10.1073/pnas.1016295107
发表时间：
2010-12-14
影响因子：
11.1
作者：
Hao, Weilong;Richardson, Aaron O.;Palmer, Jeffrey D.
通讯作者：
Palmer, Jeffrey D.
影响因子：
5.4
作者：
Boni, Maciei F.;Zhou, Yang;Holmes, Edward C.
通讯作者：
Holmes, Edward C.
影响因子：
10.7
作者：
Drouin, G;Prat, F;Clarke, GDP
通讯作者：
Clarke, GDP