Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs)
Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs)
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冷冻保存的人外周血单核细胞 (PBMC) 线粒体呼吸的测量
DOI：
10.1007/978-1-0716-2395-4_24
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Matsuzazki Hideo
中科院分区：
文献类型：
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作者：
Iwata Keiko;Xie Min-Jue;Guest Paul C.;Hirai Takaharu;Matsuzazki Hideo
由传染性病原体（如SARS-CoV-2病毒）引起的炎症性疾病可导致还原-氧化（REDOX）平衡受损和线粒体功能破坏。外周血单核细胞（PBMC）为研究炎性疾病对线粒体功能的影响提供了有用的模型，但可能受到在测定前通过冷冻保存储存这些细胞的需要的限制。在这里，我们描述了一种方法，用于改善和确定PBMC的活力与正常化的值的活细胞的数量。该方法不仅可以应用于来源于患有以改变的炎症反应为标志的疾病（如病毒感染）的患者的PBMC样品。
Inflammatory diseases caused by infectious agents such as the SARS-CoV-2 virus can lead to impaired reductive-oxidative (REDOX) balance and disrupted mitochondrial function. Peripheral blood mononuclear cells (PBMCs) provide a useful model for studying the effects of inflammatory diseases on mitochondrial function but can be limited by the need to store these cells by cryopreservation prior to assay. Here, we describe a method for improving and determining PBMC viability with normalization of values to number of living cells. The approach can be applied not only to PBMC samples derived from patients with diseases marked by an altered inflammatory response such as viral infections.