Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates
Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates
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DOI：
10.3389/fchem.2018.00519
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发表时间：
2018-11
影响因子：
5.5
通讯作者：
N. Samart;Zeyad Arhouma;Santosh Kumar;Heide A. Murakami;D. Crick;D. Crans
中科院分区：
文献类型：
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作者：
N. Samart;Zeyad Arhouma;Santosh Kumar;Heide A. Murakami;D. Crick;D. Crans
51 V NMR光谱被用来记录，使用形态分析，一个oxavanate是一个更有效的生长抑制剂的两个分枝杆菌菌株比其他oxovanadates，从而证明其与细胞的相互作用的选择性。从历史上看，草酸盐在生物学和医学研究中有许多应用，包括核糖体X射线晶体学中的相位问题研究。研究了不同钒酸盐对耻垢分枝杆菌生长的影响。smeg）和结核分枝杆菌（M. TB）进行了研究，发现形态形成对于所观察到的生长抑制是关键的。具体地说，大橙色十钒酸钠（V10 O286-）阴离子被发现是一个更强的抑制剂的生长的两个分枝杆菌物种比无色的氧钒酸钠制备偏钒酸钠。使用51 V NMR光谱和形态计算监测生长介质中的钒（V）形态和生长条件下的物种之间的转化。在这项工作中提出的研究结果是特别重要的，在考虑许多应用的聚氧乙烯醚在生物和医学研究，如X射线晶体学的核糖体的相位问题的调查。这项工作中提出的研究结果调查了草酸盐与其他生物系统的相互作用。
51V NMR spectroscopy is used to document, using speciation analysis, that one oxometalate is a more potent growth inhibitor of two Mycobacterial strains than other oxovanadates, thus demonstrating selectivity in its interaction with cells. Historically, oxometalates have had many applications in biological and medical studies, including study of the phase-problem in X-ray crystallography of the ribosome. The effect of different vanadate salts on the growth of Mycobacterium smegmatis (M. smeg) and Mycobacterium tuberculosis (M. tb) was investigated, and speciation was found to be critical for the observed growth inhibition. Specifically, the large orange-colored sodium decavanadate (V10O286-) anion was found to be a stronger inhibitor of growth of two mycobacterial species than the colorless oxovanadate prepared from sodium metavanadate. The vanadium(V) speciation in the growth media and conversion among species under growth conditions was monitored using 51V NMR spectroscopy and speciation calculations. The findings presented in this work is particularly important in considering the many applications of polyoxometalates in biological and medical studies, such as the investigation of the phase-problem in X-ray crystallography for the ribosome. The findings presented in this work investigate the interactions of oxometalates with other biological systems.