Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short-term exposure to tobacco smoke.
Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short-term exposure to tobacco smoke.
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短期暴露于烟草烟雾后，将核磷蛋白鉴定为小鼠肺组织中的新型烟草标记。
DOI：
10.1016/j.fob.2014.08.002
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发表时间：
2014
期刊：
影响因子：
2.6
通讯作者：
Ito, Takaaki
中科院分区：
文献类型：
--
作者：
Niimori-Kita, Kanako;Ogino, Kiyoshi;Mikami, Sayaka;Kudoh, Shinji;Koizumi, Daikai;Kudoh, Noritaka;Nakamura, Fumiko;Misumi, Masahiro;Shimomura, Tadasuke;Hasegawa, Koki;Usui, Fumihiko;Nagahara, Noriyuki;Ito, Takaaki
我们分析了烟草烟雾暴露激活的核磷蛋白表达。在1天和7天暴露组中鉴定了253种磷蛋白。其中，33个在对照组和暴露组中显著差异表达。鉴定出的蛋白质与炎症、应激反应和尼古丁有关。OSF 3和血影蛋白β链被确定为候选的烟草烟雾标记物。吸烟是肺部疾病的危险因素，包括慢性阻塞性肺病和肺癌。然而，介导这些疾病进展的分子机制仍不清楚。因此，我们试图通过使用暴露于烟草烟雾的小鼠肺组织的磷酸化蛋白质组学分析来分析核磷蛋白表达，从而鉴定烟草烟雾暴露激活的信号通路。将16只小鼠暴露于烟草烟雾1或7天，并通过质谱分析磷酸化肽的表达。共鉴定了253种磷蛋白，包括1天暴露组中的FACT复合物亚基SPT 16、7天暴露组中的1型角蛋白细胞骨架18（K18）和脂肪细胞脂肪酸结合蛋白，以及两组中的过氧化物氧还蛋白-1（OSF 3）和血影蛋白β链脑1（SPTBN 1）。半定量分析所确定的磷蛋白显示，33个蛋白质显着差异表达之间的控制和曝光组。根据其生物学功能对鉴定的磷蛋白进行了分类。我们发现，所鉴定的蛋白质与炎症、再生、修复、增殖、分化、形态发生以及对应激和尼古丁的反应有关。总之，我们确定了蛋白质，包括OSF 3和SPTBN 1，作为候选烟草烟雾暴露标志物;我们的研究结果提供了深入了解烟草烟雾诱导的疾病的机制。
We analyzed nuclear phosphoprotein expression activated by tobacco smoke exposure. 253 phosphoproteins were identified in 1-day and 7-day exposure groups. Of these, 33 were significantly differentially expressed in control and exposed groups. Identified proteins were related to inflammation, response to stress and nicotine. OSF3 and spectrin β chain were identified as candidate tobacco smoke markers. Smoking is a risk factor for lung diseases, including chronic obstructive pulmonary disease and lung cancer. However, the molecular mechanisms mediating the progression of these diseases remain unclear. Therefore, we sought to identify signaling pathways activated by tobacco-smoke exposure, by analyzing nuclear phosphoprotein expression using phosphoproteomic analysis of lung tissue from mice exposed to tobacco smoke. Sixteen mice were exposed to tobacco smoke for 1 or 7 days, and the expression of phosphorylated peptides was analyzed by mass spectrometry. A total of 253 phosphoproteins were identified, including FACT complex subunit SPT16 in the 1-day exposure group, keratin type 1 cytoskeletal 18 (K18), and adipocyte fatty acid-binding protein, in the 7-day exposure group, and peroxiredoxin-1 (OSF3) and spectrin β chain brain 1 (SPTBN1), in both groups. Semi-quantitative analysis of the identified phosphoproteins revealed that 33 proteins were significantly differentially expressed between the control and exposed groups. The identified phosphoproteins were classified according to their biological functions. We found that the identified proteins were related to inflammation, regeneration, repair, proliferation, differentiation, morphogenesis, and response to stress and nicotine. In conclusion, we identified proteins, including OSF3 and SPTBN1, as candidate tobacco smoke-exposure markers; our results provide insights into the mechanisms of tobacco smoke-induced diseases.
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影响因子：
4.7
作者：
Gebel, S;Gerstmayer, B;Müller, T
通讯作者：
Müller, T
影响因子：
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作者：
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亚长期暴露于主流烟草烟雾的小鼠肺部白细胞介素 6/信号转导器和转录途径激活剂的诱导。
影响因子：
2.7
作者：
Halappanavar, Sabina;Russell, Marsha;Stampfli, Martin R.;Williams, Andrew;Yauk, Carole L.
通讯作者：
Yauk, Carole L.