Identification of hsa_circ_0005654 as a new early biomarker of gastric cancer
Identification of hsa_circ_0005654 as a new early biomarker of gastric cancer
复制标题
鉴定 hsa_circ_0005654 作为胃癌新的早期生物标志物
DOI：
10.3233/cbm-190561
复制
发表时间：
2019-01-01
影响因子：
3.1
通讯作者：
Guo, Junming
中科院分区：
文献类型：
--
作者：
Wang, Yezhao;Xu, Suyuan;Guo, Junming
胃癌是世界上最常见的癌症之一。然而，目前的医学技术还没有找到一种可靠的方法来治愈晚期胃癌，早期胃癌很难诊断。因此，我们专注于已被证明参与胃癌发生的环状RNA（circRNA）。我们首先使用定量逆转录-聚合酶链反应（qRT-PCR）来评估hsa_circ_0005654在301个组织中的表达水平，包括122个健康胃粘膜样本，68个通过粘膜下剥离获得的早期胃癌和邻近非肿瘤粘膜的配对组织，以及43个慢性胃炎组织。然后，我们分析了hsa（-）circ（-）0005654的表达水平与早期胃癌患者临床病理特征的关系。通过绘制受试者工作特征（ROC）曲线和比较ROC曲线下面积（AUC），最终证实hsa（-）circ（-）0005654在早期胃癌组织中的临床诊断价值。同时，hsa_circ_0005654在早期胃癌组织中的表达水平也明显低于慢性胃炎组织（P < 0.001）。早期胃癌组织与配对正常癌旁粘膜和早期胃癌与健康对照的AUC分别为0.927和0.924。这些结果清楚地表明，hsa_circ_0005654可能作为一个新的和有前途的诊断生物标志物筛查早期胃癌。hsa_circ_0005654的AUC、敏感性和特异性均显著高于现有的胃癌相关生物标志物。
Gastric cancer is one of the most common cancers in the world. However, current medical technologies have not identified a reliable method to cure advanced gastric cancer, and early gastric cancer is difficult to diagnose. Therefore, we focused on circular RNAs (circRNAs) that have been proven to be involved in the carcinogenesis of gastric cancer. We first used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to evaluate the expression levels of hsa_circ_0005654 in 301 tissues, including 122 healthy gastric mucosa samples, 68 paired tissues from early gastric cancer and adjacent nontumor mucosae obtained by submucosal dissection, and 43 chronic gastritis tissues. Then, we analyzed the relationship between the expression levels of hsa(-)circ(-)0005654 and the clinicopathological characteristics of patients with early gastric cancer. We ultimately confirmed the clinical diagnostic value of hsa(-)circ(-)0005654 through generating receiver operating characteristic (ROC) curves and comparing the areas under the ROC curves (AUCs).Our data revealed that hsa_circ_0005654 was significantly downregulated in early gastric cancer tissues compared with matched normal mucosae (P < 0.001). Meanwhile, the expression levels of hsa_circ_0005654 in early gastric cancer tissues were also obviously lower than those in chronic gastritis tissues (P < 0.001). The AUCs of early gastric cancer tissues vs. paired normal adjacent mucosae, and that of early gastric cancer vs. healthy controls, were 0.927 and 0.924, respectively. These results clearly demonstrated that hsa_circ_0005654 may serve as a new and promising diagnostic biomarker for screening early gastric cancer. The AUC, sensitivity and specificity of hsa_circ_0005654 are significantly higher than those of present gastric cancer associated-biomarkers.