INDUCTION OF SPECIFIC IMMUNOGLOBULIN-A IN THE SMALL-INTESTINE, COLON-RECTUM, AND VAGINA MEASURED BY A NEW METHOD FOR COLLECTION OF SECRETIONS FROM LOCAL MUCOSAL SURFACES
INDUCTION OF SPECIFIC IMMUNOGLOBULIN-A IN THE SMALL-INTESTINE, COLON-RECTUM, AND VAGINA MEASURED BY A NEW METHOD FOR COLLECTION OF SECRETIONS FROM LOCAL MUCOSAL SURFACES
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DOI：
10.1128/iai.62.1.15-23.1994
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发表时间：
1994-01-01
影响因子：
3.1
通讯作者：
NEUTRA, MR
中科院分区：
文献类型：
--
作者：
HANEBERG, B;KENDALL, D;NEUTRA, MR
为了研究通过不同途径粘膜免疫小鼠后局部抗体应答的模式，开发了一种直接从粘膜表面收集分泌物的方法。在第0、10、17和24天通过将霍乱毒素施用到口腔、胃、结肠-直肠或阴道中来免疫小鼠。在第32天处死时，将吸收芯置于口腔中，并在冲洗腔内容物后通过施用管进入阴道和远端结肠-直肠以及沿着整个小肠。从灯芯中定量提取蛋白质，并通过酶联免疫吸附测定法测定特异性抗霍乱毒素免疫球蛋白A（伊加）和IgG。免疫途径对不同粘膜部位分泌物中特异性伊加的浓度有显著影响。口服免疫有效地诱导唾液中的伊加，和胃内途径是最佳的诱导伊加在小肠中。高水平的特异性伊加出现在结肠直肠粘膜表面后，直肠交付的抗原。口服、胃和直肠免疫也在阴道中产生远距离反应。然而，阴道免疫后，既没有本地也没有检测到远处的伊加反应。这些结果表明，用于保护结肠直肠和阴道粘膜表面的疫苗最好通过直肠途径接种。
In order to study patterns of local antibody responses following mucosal immunization of mice via different routes, a method for collection of secretions directly from mucosal surfaces was developed. Mice were immunized on days 0, 10, 17, and 24 by administration of cholera toxin into the oral cavity, stomach, colon-rectum, or vagina. At sacrifice on day 32, absorbent wicks were placed in the oral cavity and, via an applicator tube, into the vagina and distal colon-rectum and along the entire small intestine after flushing of luminal contents. Protein was quantitatively extracted from wicks, and specific anti-cholera toxin immunoglobulin A (IgA) and IgG were measured by enzyme-linked immunosorbent assay. Concentrations of specific IgA in secretions at various mucosal sites were dramatically influenced by the route of immunization. Oral immunization effectively induced IgA in saliva, and the intragastric route was optimal for induction of IgA in the small intestine. High levels of specific IgA appeared on the colonic-rectal mucosal surface only after rectal delivery of antigen. Oral, gastric, and rectal immunizations also produced distant responses in the vagina. Following vaginal immunization, however, neither local nor distant IgA responses were detected. These results suggest that vaccines intended for protection of colonic-rectal and vaginal mucosal surfaces might best be administered by the rectal route.