Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye
Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye
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DOI：
10.1007/s10529-008-9817-z
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发表时间：
2008-12-01
影响因子：
2.7
通讯作者：
Zhang, Chenyun
中科院分区：
文献类型：
--
作者：
Guo, Mei;Lu, Fuping;Zhang, Chenyun
在确定的培养基中组成型地产生来自甲醇毕赤酵母中的花斑栓菌的重组漆酶。使用超滤、阴离子交换层析和凝胶过滤纯化重组漆酶。通过 SDS-PAGE 估计纯化漆酶的分子量为 64 kDa。当使用25 U漆酶ml(-1)时，在45℃和pH 5下16小时后，纯化的重组漆酶最初在80 mg l(-1) 下对Remazol Brilliant Blue R (RBBR)脱色超过90%。纯化的重组漆酶无需额外的氧化还原介体即可有效脱色RBBR。
A recombinant laccase from Trametes versicolor in Pichia methanolica was produced constitutively in a defined medium. The recombinant laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration. The molecular weight of the purified laccase was estimated as 64 kDa by SDS-PAGE. The purified recombinant laccase decolorized more than 90% of Remazol Brilliant Blue R (RBBR) initially at 80 mg l(-1) after 16 h at 45 degrees C and pH 5 when 25 U laccase ml(-1) was used. The purified recombinant laccase could efficiently decolorize RBBR without additional redox mediators.