Heterogeneous nanostructural and nanoelastic properties of pericellular and interterritorial matrices of chondrocytes by atomic force microscopy
Heterogeneous nanostructural and nanoelastic properties of pericellular and interterritorial matrices of chondrocytes by atomic force microscopy
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DOI：
10.1016/j.jsb.2003.10.003
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发表时间：
2004-03-01
影响因子：
3
通讯作者：
Mao, JJ
中科院分区：
文献类型：
--
作者：
Allen, DM;Mao, JJ
透明软骨由稀疏的软骨细胞和丰富的细胞外基质组成。有一个缺乏的实验数据支持的概念，可以想象的区域差异的机械性能的chonelomatrium矩阵。在视觉分化的细胞周围和interterritorial矩阵在每个19个新鲜的生长板样品与甲苯胺蓝和茜素红标签，纳米压痕分别应用于细胞周围的矩阵和interterritorial矩阵，使用液相原子力显微镜和实时成像。interterritorial矩阵表现出细长的平行脊，而细胞周围的矩阵表现出不规则的，短距离的隆起特征孔和运河。600 nm（2）扫描尺寸下的表面轮廓分析显示，区域间基质的表面粗糙度（71+/-18 nm;平均值+/-SE）显著大于细胞周围基质（24+/-4 nm）（P
Hyaline cartilage consists of sparse chondrocytes and abundant extracellular matrix. There is a paucity of experimental data in support of the notion of conceivable regional differences in the mechanical properties of chondral matrices. Upon visual differentiation of the pericellular and interterritorial matrices in each of 19 fresh growth plate samples with toluidine blue and alizarin red labels, nanoindentation was applied separately to the pericellular matrix and interterritorial matrix to using fluid-phase atomic force microscopy and real-time imaging. The interterritorial matrix demonstrated elongated parallel ridges, whereas the pericellular matrix showed irregular, short-range elevations with characteristic pores and canals. Analysis of surface contours at 600 nm(2) scan size revealed that the interterritorial matrix had significantly greater surface roughness (71+/-18 nm; mean+/-SE) than the pericellular matrix (24+/-4nm) (P