Chemistry and Toxicity of Sediments from San Diego Bay, Including a Biomarker (P450 RGS) Response
Chemistry and Toxicity of Sediments from San Diego Bay, Including a Biomarker (P450 RGS) Response
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DOI：
10.1520/stp11699s
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发表时间：
1996-12
影响因子：
3.2
通讯作者：
JW Anderson;F. Newton;J. Hardin;Rh Tukey;K. Richter
中科院分区：
文献类型：
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作者：
JW Anderson;F. Newton;J. Hardin;Rh Tukey;K. Richter
从圣地亚哥湾的海军对接设施附近收集了30个沉积物样品，用于进行片足类动物、牡蛎幼虫、Microtox和一种称为细胞色素P450报告基因系统（RGS）的新的快速筛选试验的生物测定。这种来自人类肝癌细胞的RGS细胞系经过改造，当染色体上的CYP1A1基因被有毒和致癌的有机物（二恶英，共面PCB，PAH）诱导时，可以产生荧光素酶。用Microtox和牡蛎幼虫测试了洗脱液，用Microtox和P450 RGS测定法测试了沉积物的有机提取物。化学分析包括总有机碳（TOC）和酸挥发性硫化物（AVS）沿着以及各种金属和有机化学品。同时提取的金属（SEM）AVS的比例进行了比较，牡蛎幼虫和端足类动物的毒性反应。沿着每个桥墩取样，污染物浓度随着距离海岸的距离而降低。对所有生物和化学数据进行相关矩阵分析。化学测量和生物反应之间的最强相关性是总PAH与P450 RGS反应。使用P450 RGS作为筛选工具来评估沉积物上更多污染物的相对风险具有生物学意义，并且是确定哪些样品需要完整化学表征的快速且廉价的方法。«少
Thirty sediment samples were collected from the vicinity of the Naval Docking Facility in San Diego Bay and used to conduct bioassays with amphipods, oyster larvae, Microtox, and a new rapid screening test called the cytochrome P450 Reporter Gene System (RGS). This RGS cell line, from a human liver cancer cell, has been engineered to produce luciferase, when the CYP1A1 gene on the chromosome is induced by toxic and carcinogenic organics (dioxin, coplanar PCBs, PAHs). Elutriates were tested with both Microtox and oyster larvae, and organic extracts of sediments were tested with Microtox and the P450 RGS assay. Chemical analyses included total organic carbon (TOC), and acid volatile sulfides (AVS) along with a wide range of metals and organic chemicals. The simultaneously extracted metals (SEM) to AVS ratio was compared to the toxic response of oyster larvae and amphipods. Along each of the piers sampled, contaminant concentrations decreased with distance from shore. A correlation matrix analysis of all biological and chemical data was conducted. The strongest correlation between a chemical measurement and a biological response was that of total PAH versus the P450 RGS response. The use of P450 RGS as a screening tool to assess the relative risk ofmore » contaminants on sediments is biologically meaningful, and is a rapid and inexpensive means of determining which samples require complete chemical characterization.« less