ISOPRENOID SYNTHESIS IN ESCHERICHIA-COLI - SEPARATION AND PARTIAL-PURIFICATION OF 4 ENZYMES INVOLVED IN THE SYNTHESIS
ISOPRENOID SYNTHESIS IN ESCHERICHIA-COLI - SEPARATION AND PARTIAL-PURIFICATION OF 4 ENZYMES INVOLVED IN THE SYNTHESIS
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DOI：
10.1093/oxfordjournals.jbchem.a135600
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发表时间：
1986-05-01
影响因子：
2.7
通讯作者：
KATSUKI, H
中科院分区：
文献类型：
--
作者：
FUJISAKI, S;NISHINO, T;KATSUKI, H
用DEAE-Toyoptylase层析法从大肠杆菌中部分纯化了异戊烯焦磷酸（IPP）异构酶、法尼基焦磷酸（FPP）合成酶、八异戊二烯焦磷酸（OPP）合成酶和十一异戊二烯焦磷酸（UPP）合成酶。FPP合成酶催化IPP与焦磷酸二甲基烯丙酯（DPP）以及焦磷酸香叶酯（GPP）缩合生成最终产物FPP。C45合成酶和UPP合成酶催化IPP与FPP缩合，分别产生顺式和顺式，反式焦磷酸聚异戊二烯酯（C45-，C50-和C55-化合物）。DPP和GPP都不作为任一酶的引发底物。这四种酶的活性需要Mg ~（2+）或Mn ~（2+）。UPP合成酶的活性也需要Triton X-100。TritonX-100的加入对IPP异构酶和FPP合成酶没有影响，但对FPP合成酶有促进作用。这四种酶的结合可能保证了在E.杆菌
Isopentenyl pyrophosphate (IPP) isomerase, farnesyl pyrophosphate (FPP) synthetase, octaprenyl pyrophosphate (OPP) synthetase and undecaprenyl pyrophosphate (UPP) synthetase were partially purified from Escherichia coli by DEAE-Toyopearl chromatography. FPP synthetase catalyzed the condensation of IPP with dimethylallyl pyrophosphate (DPP) as well as with geranyl pyrophoshate (GPP) to yield FPP as final product. OPP synthetase and UPP synthetase catalyzed the condensation of IPP with FPP to yield OPP and cis,trans-polyprenyl pyrophosphates (the C45-, C50-, and C55-compound), respectively. Neither DPP nor GPP acted as a priming substrate for either enzyme. These four enzymes required Mg2+ or Mn2+ for their activities. UPP synthetase required also Triton X-100 for its activity. The addition of Triton X-100 enhanced OPP synthetase, but it did not affect IPP isomerase and FPP synthetase. It seems possible that the combination of the four enzymes ensures the in vivo synthesis of long-chain isoprenoids in E. coli.