Localization of plasma membrane and secretory calcium pumps in the mammary gland
Localization of plasma membrane and secretory calcium pumps in the mammary gland
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DOI：
10.1016/j.bbrc.2008.03.003
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发表时间：
2008-05-09
影响因子：
3.1
通讯作者：
Monteith, Gregory R.
中科院分区：
文献类型：
--
作者：
Faddy, Helen M.;Smart, Chanel E.;Monteith, Gregory R.
直到最近，人们还认为牛奶富含钙的机制几乎完全是通过分泌途径实现的。然而，最近的研究表明，质膜钙ATPase PMCA2是钙转运到牛奶中的主要机制，突出了顶端钙转运的主要作用。我们比较了最近发现的分泌性钙ATPase SPCA2和SPCA1在小鼠乳腺发育过程中的表达。在哺乳期间，SPCA2的表达水平增加了35倍以上，表达局限于腔分泌细胞，而SPCA1的表达仅增加了2倍，并在整个乳腺细胞中表达。我们还观察到PMCA2和PMCA1在定位上的重大差异。我们的研究强调了PMCA2和SPCA2在哺乳中可能的特定作用，并表明钙进入乳汁的运输是顶端和分泌途径之间的复杂相互作用。(C)2008 Elsevier Inc.保留所有权利。
Until recently the mechanism for the enrichment of milk with calcium was thought to be almost entirely via the secretory pathway. However, recent studies suggest that a plasma membrane calcium ATPase, PMCA2, is the primary mechanism for calcium transport into milk, highlighting a major role for apical calcium transport. We compared the expression of the recently identified secretory calcium ATPase, SPCA2, and SPCA1, in the mouse mammary gland during development. SPCA2 levels increased over 35-fold during lactation with expression localized to luminal secretory cells, while SPCA1 increased only a modest 2-fold and was expressed throughout the cells of the mammary gland. We also observed major differences in the localization of PMCA2 and PMCA1. Our studies highlight the likely specific roles of PMCA2 and SPCA2 in lactation and indicate that calcium transport into milk is a complex interplay between apical and secretory pathways. (c) 2008 Elsevier Inc. All rights reserved.