Kamiuntanto increases prefrontal extracellular serotonin levels and ameliorates depression-like behaviors in mice
Kamiuntanto increases prefrontal extracellular serotonin levels and ameliorates depression-like behaviors in mice
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DOI：
10.1016/j.jphs.2018.11.008
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发表时间：
2019-02-01
影响因子：
3.5
通讯作者：
Yabe，Takeshi
中科院分区：
文献类型：
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作者：
Hiraki，Yosuke;Araki，Ryota;Yabe，Takeshi
Kamiuntanto（KUT;拼音为Jia Wei Wen Dan Tang）是一种传统的日本汉方药物，用于治疗心理功能障碍。然而，KUT的作用机制尚不清楚。为了研究KUT改善特性的机制，研究了KUT对隔离饲养小鼠异常行为和前额叶单胺能系统的影响。KUT（1000 mg/kg）可逆转隔离饲养小鼠强迫游泳试验中的运动过度和增加不动性，抗抑郁药氟西汀（30 mg/kg）也发现了这一点。与此相反，一个不完整的KUT公式排除竹（BC），一个组成草药的KUT，没有扭转异常行为的隔离饲养小鼠或增加前额叶细胞外5-HT。此外，在强迫游泳试验中，KUT的抗抑郁样作用被5-HT 1B拮抗剂GR 127935预处理所阻止。这些研究结果表明，KUT可能通过5-HTergic系统改善抑郁症状，而BC在KUT的抗抑郁样作用中起着重要作用。
Kamiuntanto (KUT; Jia Wei Wen Dan Tang in Pinyin) is a traditional Japanese Kampo medicine that is used to treat psychological dysfunction. However, the mechanisms of action of KUT are not understood. To investigate the mechanisms underlying the ameliorative properties of KUT, the effects of KUT on abnormal behaviors of isolation-reared mice and on the prefrontal monoaminergic system were examined. KUT (1000 mg/kg) reversed encounter-induced hyperactivity and increased immobility in the forced swim test in isolation-reared mice, as also found for an antidepressant, fluoxetine (30 mg/kg).In vivomicrodialysis showed that KUT (1000 mg/kg) transiently increased the level of extracellular serotonin (5-HT) in the prefrontal cortex. In contrast, an incomplete KUT formula excluding Bambusae Caulis (BC), a component herb of KUT, did not reverse abnormal behaviors of isolation-reared mice or increase prefrontal extracellular 5-HT. Furthermore, the antidepressant-like effect of KUT in the forced swim test was prevented by pretreatment with GR127935, a 5-HT1Bantagonist. These findings suggest that KUT may ameliorate depressive symptoms via 5-HTergic systems, and that BC plays an important role in the antidepressant-like effects of KUT.