Linkage and association mapping of ovule number per ovary (ON) in oilseed rape (Brassica napus L.)
Linkage and association mapping of ovule number per ovary (ON) in oilseed rape (Brassica napus L.)
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DOI：
10.1007/s11032-023-01355-7
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发表时间：
2023-02-01
影响因子：
3.1
通讯作者：
Hong，Dengfeng
中科院分区：
文献类型：
--
作者：
Ahmad，Ali;Li，Wenhui;Hong，Dengfeng
在花发育过程中产生的胚珠数（ON）决定了每角果的最大种子数，从而影响作物生产力;然而，在油菜（Brassica napus）中ON的遗传基础仍然知之甚少。在这项研究中，我们从遗传学角度剖析了双单倍体（DH）群体和自然群体（NP）中的ON变异。表型分析表明，两个群体的ON均呈正态分布，DH群体和自然群体的广义遗传力分别为0.861和0.930。连锁分析共检测到5个与ON相关的QTL，分别为qON-A03、qON-A07、qON-A07 - 2、qON-A10和qON-C06。全基因组关联研究（GWAS）分别使用单位点模型GLM和多位点模型MrMLM和FASTMrMLM发现了214、48和40个显著的单核苷酸多态性（SNP）。这些QTL和SNPs对表型变异的解释率（PVE）分别为2.00 - 17.40%和5.03 - 7.33%。整合两种策略的结果，从染色体A03、A07和A10中鉴定出与ON相关的四个共有基因组区域。本研究结果初步揭示了ON的遗传基础，为水稻产量改良提供了有用的分子标记。油菜。
Ovule number (ON) produced during flower development determines the maximum number of seeds per silique and thereby affects crop productivity; however, the genetic basis of ON remains poorly understood in oilseed rape (Brassica napus). In this study, we genetically dissected the ON variations in a double haploid (DH) population and in natural population (NP) by linkage mapping and genome-wide association analysis. Phenotypic analysis showed that ON displayed normal distribution in both populations with the broad-sense heritability of 0.861 (DH population) and 0.930 (natural population). Linkage mapping identified 5 QTLs related to ON, includingqON-A03,qON-A07,qON-A07-2,qON-A10, andqON-C06. Genome-wide association studies (GWAS) revealed 214, 48, and 40 significant single-nucleotide polymorphisms (SNPs) by individually using the single-locus model GLM and the multiple-locus model MrMLM and FASTMrMLM. The phenotypic variation explained (PVE) by these QTLs and SNPs ranged from 2.00–17.40% to 5.03–7.33%, respectively. Integration of the results from both strategies identified four consensus genomic regions associated with ON from the chromosomes A03, A07, and A10. Our results preliminarily resolved the genetic basis of ON and provides useful molecular markers for plant yield improvement inB. napus.