Trefoil peptide expression and goblet cell number in rat intestine:: effects of KGF and fasting-refeeding
Trefoil peptide expression and goblet cell number in rat intestine:: effects of KGF and fasting-refeeding
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DOI：
10.1152/ajpregu.00428.2002
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发表时间：
2003-02-01
影响因子：
2.8
通讯作者：
Ziegler, TR
中科院分区：
文献类型：
--
作者：
Fernández-Estívariz, C;Gu, LH;Ziegler, TR
三叶因子家族肽TFF 1、TFF 2和TFF 3对于肠粘膜保护和恢复是重要的。角质细胞生长因子（KGF）刺激上皮细胞的增殖和分化，对杯状细胞具有强有力的作用。为了研究食物摄入和KGF之间的相互作用，大鼠自由进食（对照），禁食72小时，或禁食72小时，然后再进食72小时，有或没有KGF（3 mg.kg（-1）.day（-1））。随着禁食时间的延长，十二指肠杯状细胞数量增加，十二指肠和空肠TFF 3 mRNA表达减少，TFF 3蛋白表达无变化或增加。KGF在禁食刺激结肠生长，正常化TFF 3 mRNA在十二指肠和空肠，并广泛上调肠杯状细胞数量和TFF 3蛋白表达。随着禁食再喂养，KGF增加小肠和结肠粘膜的生长，杯状细胞数量，和TFF 3蛋白，但对TFF 3 mRNA有不同的影响。KGF诱导TFF 2 mRNA和蛋白在十二指肠和空肠的营养方案。我们的结论是，营养的可用性修改大鼠肠道杯状细胞的数量，TFF 3 mRNA，并在特定区域的方式肠道营养作用的KGF。KGF增强近端小肠中的TFF 2表达，并增加整个肠道中的杯状细胞数量和TFF 3蛋白含量，而与食物摄入无关。
The trefoil factor family peptides TFF1, TFF2, and TFF3 are important for gut mucosal protection and restitution. Keratinocyte growth factor (KGF) stimulates proliferation and differentiation of epithelial cells with potent effects on goblet cells. To investigate interactions between food intake and KGF, rats were fed ad libitum (control), fasted for 72 h, or fasted for 72 h and then refed for 72 h with or without KGF (3 mg.kg(-1).day(-1)). With fasting, goblet cell number in duodenum increased, TFF3 mRNA in duodenum and jejunum decreased, and TFF3 protein did not change or increased. KGF during fasting stimulated colonic growth, normalized TFF3 mRNA in duodenum and jejunum, and broadly upregulated gut goblet cell number and TFF3 protein expression. With fasting-refeeding, KGF increased small bowel and colonic mucosal growth, goblet cell number, and TFF3 protein but had variable effects on TFF3 mRNA. KGF induced TFF2 mRNA and protein in duodenum and jejunum with both nutritional regimens. We conclude that nutrient availability modifies rat intestinal goblet cell number, TFF3 mRNA, and the gut-trophic effects of KGF in a region-specific manner. KGF enhances TFF2 expression in proximal small bowel and increases goblet cell number and TFF3 protein content throughout the intestine independent of food intake.