Over-expression of ArathEULS3 confers ABA sensitivity and drought tolerance in Arabidopsis
Over-expression of ArathEULS3 confers ABA sensitivity and drought tolerance in Arabidopsis
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ArathEULS3 的过表达赋予拟南芥 ABA 敏感性和耐旱性
DOI：
10.1007/s11240-014-0453-0
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发表时间：
2014-06-01
影响因子：
3
通讯作者：
Yang, Yi
中科院分区：
文献类型：
--
作者：
Li, Dekuan;Wang, Xiaoyu;Yang, Yi
脱落酸（阿坝）是一种重要的植物激素，参与调节植物的生长、发育和对各种环境挑战的适应。阿坝受体1（RCAR 1，也称为PYL 9）是拟南芥中新发现的阿坝受体。为了鉴定RCAR 1的相互作用伙伴，我们进行了酵母双杂交筛选。鉴定了一种蛋白质，ArathEULS 3，其属于植物凝集素的Eurobuseuropaeus凝集素（EUL）家族。通过GST pull-down分析证实了RCAR 1与ArathEULS 3之间的相互作用。RCAR 1-EGFP和ArathEULS 3-EGFP在拟南芥原生质体中的瞬时表达结果表明，这两种蛋白主要在细胞质和细胞核中表达。真实的时间qRT-PCR分析显示RCAR 1的过表达增加ArathEULS 3的表达。此外，在拟南芥中上调ArathEULS 3赋予阿坝超敏性在萌发后生长和增强耐旱性，但不影响RD 29 B，RAB 18和RD 29 A（阿坝和干旱响应基因）的表达。以前，ArathEULS 3被证明是一种碳水化合物结合的植物凝集素。因此，我们的研究结果揭示了非生物胁迫反应和植物凝集素之间的直接联系。
Abscisic acid (ABA) is an important phytohormone involved in the regulation of plant growth, development and adaption to various environmental challenges. Regulatory component of ABA receptor 1 (RCAR1, also known as PYL9) acts as a newly discovered ABA receptor in Arabidopsis. To identify interacting partners of RCAR1, we have carried out a yeast two-hybrid screen. One protein was identified, ArathEULS3, which belongs to the Euonymus europaeus lectin (EUL) family of plant lectins. The interaction between RCAR1 and ArathEULS3 was confirmed by GST pull-down assay. Transient expression of RCAR1-EGFP and ArathEULS3-EGFP in Arabidopsis protoplasts revealed that both proteins were mainly expressed in cytoplasm and nucleus. Real time qRT-PCR analysis showed that over-expression of RCAR1 increased the expression of ArathEULS3. Furthermore, up-regulating ArathEULS3 in Arabidopsis conferred ABA hypersensitivity during post-germination growth and enhanced drought tolerance, but did not affect the expression of RD29B, RAB18 and RD29A (ABA- and drought-responsive genes). Previously, ArathEULS3 was shown as a carbohydrate-binding plant lectin. Thus, our results reveal a direct connection between abiotic stress responses and plant lectin.