CADMIUM UPTAKE AND DISTRIBUTION IN MOUSE EMBRYOS - FOLLOWING MATERNAL EXPOSURE DURING THE ORGANOGENIC PERIOD - A SCINTILLATION AND AUTORADIOGRAPHIC STUDY
CADMIUM UPTAKE AND DISTRIBUTION IN MOUSE EMBRYOS - FOLLOWING MATERNAL EXPOSURE DURING THE ORGANOGENIC PERIOD - A SCINTILLATION AND AUTORADIOGRAPHIC STUDY
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DOI：
10.1002/tera.1420270304
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
WEBSTER, WS
中科院分区：
文献类型：
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作者：
CHRISTLEY, J;WEBSTER, WS
对怀孕的 C57BL/6J 小鼠进行单次腹膜内注射。在妊娠第 9 天注射致畸剂量 (2400 μg Cd/kg)、中间剂量 (40 μg Cd/kg) 或痕量剂量 (0.66 μg Cd/kg) CdCl2；每个剂量含有 109CdCl2 (20 μCi)。在暴露于镉后的不同时间对小鼠实施安乐死，并在伽马计数器中测定胚胎中镉的含量。对于所有 3 个剂量，快速进入胚胎的剂量百分比虽低但相似；然后，该水平在接下来的 11 小时内下降，仅在 24 小时内增加，并在怀孕的剩余时间内继续上升。当镉含量与胚胎重量相关时，镉浓度在1小时后达到最高水平，然后迅速下降，并在妊娠的剩余时间内持续下降。放射自显影研究表明，致畸剂量的镉进入了胚胎的所有组织，但特别集中在神经管、肢芽和肠道的细胞中。 12 小时时，胚胎中出现细胞损伤，但受影响的细胞不一定是重度标记的细胞。在暴露于非致畸剂量的胚胎中，在胚胎肠道和肢芽外胚层中观察到最高的镉积累，但所有组织均显示出低水平的标记。
Pregnant C57BL/6J mice were given a single i.p. injection of either a teratogenic (2400 .mu.g Cd/kg), an intermediate (40 .mu.g Cd/kg) or a trace dose (0.66 .mu.g Cd/kg) of CdCl2 on day 9 of gestation; each dose contained 109CdCl2 (20 .mu.Ci). The mice were euthanized at various times after Cd exposure, and the amount of Cd in the embryos was determined in a gamma counter. For all 3 doses a low, but similar, percentage of the dose rapidly entered the embryos; levels then decreased during the next 11 h, only to rise gain by 24 h and continue to rise for the remainder of the pregnancy. When the Cd content was related to embryonic weight the Cd concentration was at its highest level after 1 h and then decreased rapidly and continued to decrease for the rest of pregnancy. Autoradiographic studies showed that the teratogenic dose of Cd entered all tissues of the embryos but was particularly localized in cells of the neural tube, limb buds and gut. By 12 h cell damage was seen in the embryos but the affected cells were not necessarily the heavily labeled cells. In embryos exposed to the nonteratogenic dose, the highest Cd accumulation was seen in the embryonic gut and limb bud ectoderm but all tissues showed a low level of labeling.