tRNA dynamics between the nucleus, cytoplasm and mitochondrial surface: Location, location, location.
tRNA dynamics between the nucleus, cytoplasm and mitochondrial surface: Location, location, location.
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DOI：
10.1016/j.bbagrm.2017.11.007
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Hopper AK
中科院分区：
文献类型：
--
作者：
Chatterjee K;Nostramo RT;Wan Y;Hopper AK
虽然tRNAs在细胞质中参与蛋白质翻译的基本功能，但tRNA转录和许多加工步骤都发生在细胞核中。这种tRNA生物发生和功能之间的亚细胞分离要求tRNA在被称为“初级tRNA核输出”的步骤中被有效地递送到细胞质。令人惊讶的是，tRNA核质运输不是单向的，而是双向的。细胞质的tRNAs通过“tRNA逆行核导入”步骤被输入回细胞核，这是从萌芽酵母到脊椎动物细胞的保守步骤，并已被病毒(如HIV)劫持，用于人类细胞中病毒逆转录复合体的核导入。在适当的环境条件下，被输入到细胞核中的细胞质tRNA通过第三个核质穿梭步骤返回到细胞质中，这一步骤被称为tRNA核再出口，这也是从萌芽酵母到脊椎动物细胞的保守步骤。我们描述了tRNA核浆运动的3个步骤及其调控。TRNA有多种核输出和输入途径。不同的tRNA核出口蛋白似乎具有底物特异性，这导致了细胞蛋白质组可能在tRNA核出口水平上受到调控的可能性。此外，在一些生物中，如发芽酵母，前tRNA剪接异四聚体内切酶(SEN)位于线粒体的细胞质表面，它从前tRNA中移除内含子。因此，我们还描述了SEN复合体在线粒体上的定位和线粒体上前tRNA的剪接，这在tRNAs参与蛋白质翻译之前发生。本文是由Boguta Magdalena博士编辑的题为：SI：tRNA合成的调节和在生理条件和疾病中的修饰的特刊的一部分。
Although tRNAs participate in the essential function of protein translation in the cytoplasm, tRNA transcription and numerous processing steps occur in the nucleus. This subcellular separation between tRNA biogenesis and function requires that tRNAs be efficiently delivered to the cytoplasm in a step termed “primary tRNA nuclear export”. Surprisingly, tRNA nuclear-cytoplasmic traffic is not unidirectional, but, rather, movement is bidirectional. Cytoplasmic tRNAs are imported back to the nucleus by the “tRNA retrograde nuclear import” step which is conserved from budding yeast to vertebrate cells and has been hijacked by viruses, such as HIV, for nuclear import of the viral reverse transcription complex in human cells. Under appropriate environmental conditions cytoplasmic tRNAs that have been imported into the nucleus return to the cytoplasm via the 3rd nuclear-cytoplasmic shuttling step termed “tRNA nuclear re-export”, that again is conserved from budding yeast to vertebrate cells. We describe the 3 steps of tRNA nuclear-cytoplasmic movements and their regulation. There are multiple tRNA nuclear export and import pathways. The different tRNA nuclear exporters appear to possess substrate specificity leading to the tantalizing possibility that the cellular proteome may be regulated at the level of tRNA nuclear export. Moreover, in some organisms, such as budding yeast, the pre-tRNA splicing heterotetrameric endonuclease (SEN), which removes introns from pre-tRNAs, resides on the cytoplasmic surface of the mitochondria. Therefore, we also describe the localization of the SEN complex to mitochondria and splicing of pre-tRNA on mitochondria, which occurs prior to the participation of tRNAs in protein translation. This article is part of a Special Issue entitled: SI: Regulation of tRNA synthesis and modification in physiological conditions and disease edited by Dr. Boguta Magdalena.
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DOI：
10.1083/jcb.130.5.1017
发表时间：
1995-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Corbett AH;Koepp DM;Schlenstedt G;Lee MS;Hopper AK;Silver PA
通讯作者：
Silver PA
影响因子：
3.3
作者：
Allemand, E;Dokudovskaya, S;Tazi, J
通讯作者：
Tazi, J
影响因子：
56.9
作者：
Blomen, Vincent A.;Majek, Peter;Brummelkamp, Thijn R.
通讯作者：
Brummelkamp, Thijn R.
影响因子：
4.8
作者：
Boland, Coilin;Hayes, Patti;Kelly, Vincent P.
通讯作者：
Kelly, Vincent P.
影响因子：
5.3
作者：
Dekker, PJT;Ryan, MT;Pfanner, N
通讯作者：
Pfanner, N