Dielectrophoretic trapping of single bacteria at carbon nanofiber nanoelectrode arrays
Dielectrophoretic trapping of single bacteria at carbon nanofiber nanoelectrode arrays
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DOI：
10.1021/jp076346e
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发表时间：
2007-12-13
影响因子：
2.9
通讯作者：
Li, Jun
中科院分区：
文献类型：
--
作者：
Arumugam, Prabhu U.;Chen, Hua;Li, Jun
我们提出了一种交流介电电泳（DEP）技术，用于单细胞捕获使用嵌入式碳纳米管（CNF）纳米电极阵列（NEAs）。将垂直排列的碳纳米纤维镶嵌在SiO2基体中制成的NEAs在水溶液中用作“点-盖”DEP器件。电极尺寸的小型化提供了高度聚焦的电场，其梯度增强了几个数量级。这在电极表面附近产生极大的正DEP力，并捕获小的生物颗粒以抵抗强大的流体动力。这项技术有望在纳米尺度上进行新的细胞生物学实验。我们预计，这种纳米DEP设备的自下而上的方法允许在芯片实验室设备中确定性地集成数百万个纳米电极，并且通常可用于操纵亚微米颗粒。
We present an ac dielectrophoretic (DEP) technique for single-cell trapping using embedded carbon nanofiber (CNF) nanoelectrode arrays (NEAs). NEAs fabricated by inlaying vertically aligned carbon nanofibers in SiO2 matrix are applied as "points-and-lid" DEP devices in aqueous solution. The miniaturization of the electrode size provides a highly focused electrical field with the gradient enhanced by orders of magnitude. This generates extremely large positive DEP forces near the electrode surface and traps small bioparticles against strong hydrodynamic forces. This technology promises new capabilities to perform novel cell biology experiments at the nanoscale. We anticipate that the bottom-up approach of such nano-DEP devices allows the integration of millions of nanolectrodes deterministically in lab-on-a-chip devices and will be generally useful for manipulating submicron particles.