The ATPase TER94 regulates Notch signaling during Drosophila wing development
The ATPase TER94 regulates Notch signaling during Drosophila wing development
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ATPase TER94 在果蝇翅膀发育过程中调节 Notch 信号传导。
DOI：
10.1242/bio.038984
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发表时间：
2019-01-01
期刊：
影响因子：
2.4
通讯作者：
Zhang, Junzheng
中科院分区：
文献类型：
--
作者：
Li, Yunlong;Liu, Tong;Zhang, Junzheng
进化上保守的Notch信号通路在各种发育环境中发挥着至关重要的作用。 Notch 通路活性的调节涉及多种机制。通过遗传镶嵌筛选，我们发现 ATPase TER94 在果蝇翅膀发育过程中充当 Notch 信号传导的正调节因子。 TER94 的缺失会导致成虫翅膀出现边缘缺口，并导致缺口靶基因在翅膀边缘形成过程中无翼和切割减少。我们提供的证据表明，Notch 蛋白的正确定位和激活可能需要 TER94。此外，我们发现 TER94 接头 p47 的敲低会导致类似的 Notch 信号缺陷。尽管 TER94 复合物参与多种细胞过程，但其在调节 Notch 通路中的作用此前尚未被表征。我们的研究表明，TER94 正向调节 Notch 信号传导，从而揭示了 TER94 在发育中的新作用。
The evolutionarily conserved Notch signaling pathway plays crucial roles in various developmental contexts. Multiple mechanisms are involved in the regulation of Notch pathway activity. Identified through a genetic mosaic screen, we show that the ATPase TER94 acts as a positive regulator of Notch signaling during Drosophila wing development. Depletion of TER94 causes marginal notches in the adult wing and the reduction of Notch target genes wingless and cut during wing margin formation. We provide evidence that TER94 is likely required for proper Notch protein localization and activation. Furthermore, we show that knockdown of the TER94 adaptor p47 leads to similar Notch signaling defects. Although the TER94 complex is implicated in various cellular processes, its role in the regulation of Notch pathways was previously uncharacterized. Our study demonstrates that TER94 positively regulates Notch signaling and thus reveals a novel role of TER94 in development.