Cationic cholesterol-dependent LNP delivery to lung stem cells, the liver, and heart
Cationic cholesterol-dependent LNP delivery to lung stem cells, the liver, and heart
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DOI：
10.1073/pnas.2307801120
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发表时间：
2024-03
影响因子：
11.1
通讯作者：
Afsane Radmand;Hyejin Kim;Jared P Beyersdorf;Curtis N Dobrowolski;Ryan Zenhausern;Kalina Paunovska;Sebastian G. Huayamares;Xuanwen Hua;Keyi Han;David Loughrey;Marine Z. C. Hatit;Ada Del Cid;Huanzhen Ni;Aram Shajii;Andrea Li;Abinaya Muralidharan;H. Peck;Karen E Tiegreen;Shu Jia;P. Santangelo;J. Dahlman
中科院分区：
文献类型：
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作者：
Afsane Radmand;Hyejin Kim;Jared P Beyersdorf;Curtis N Dobrowolski;Ryan Zenhausern;Kalina Paunovska;Sebastian G. Huayamares;Xuanwen Hua;Keyi Han;David Loughrey;Marine Z. C. Hatit;Ada Del Cid;Huanzhen Ni;Aram Shajii;Andrea Li;Abinaya Muralidharan;H. Peck;Karen E Tiegreen;Shu Jia;P. Santangelo;J. Dahlman
将阳离子辅助脂质添加到脂质纳米颗粒（LNP）中增加肺递送并减少肝递送。然而，电荷与利比里亚国家警察目标之间的关系的普遍性仍不清楚。在这里，我们报告，阳离子胆固醇依赖性的向性可以不同于阳离子辅助脂质依赖性的向性，沿着LNP的发展，提供mRNA的心脏以及肺干细胞。这些数据提供的证据表明，电荷依赖性向性可能是有用的遗传疾病，需要交付到多个组织。
Significance Adding cationic helper lipids to lipid nanoparticles (LNPs) increases lung delivery and decreases liver delivery. However, the universality of the relationship between charge and LNP targeting remains unclear. Here, we report that cationic cholesterol-dependent tropism can differ from cationic helper lipid–dependent tropism, along with the development of an LNP that delivers mRNA to the heart as well as lung stem cells. These data provide evidence that charge-dependent tropism may be useful for genetic diseases requiring delivery to multiple tissues.