Ovalocytosis without band 3 gene 27-bp deletion and malaria infection
Ovalocytosis without band 3 gene 27-bp deletion and malaria infection
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DOI：
10.1537/ase.050802
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发表时间：
2006-08-01
影响因子：
0.7
通讯作者：
Ishida, Takafumi
中科院分区：
文献类型：
--
作者：
Kimura, Masako;Soemantri, Augustinus;Ishida, Takafumi
对疟疾的遗传适应是人类学和人类遗传学领域的一个长期研究课题。东南亚卵泡细胞增多症 (SAO) 已被证明对疟疾具有抵抗力；然而，现有数据的影响存在争议。特别是，SAO 对疟疾的抗性在 SAO 类型方面是未知的，带 3 基因 (B3 Delta 27) 中是否有 27 个碱基对缺失。为了阐明 SAO 与疟疾之间的关系，我们调查了疟疾流行的印度尼西亚东加里曼丹选定地区居民的疟原虫感染、红细胞形态以及 B3 Delta 27 的存在情况。我们在显微镜下筛查了128例外周血涂片的疟原虫感染情况和红细胞形态，然后从涂片中提取DNA作为模板进行聚合酶链式反应检测B3 Delta 27。疟原虫（包括间日疟原虫和恶性疟原虫）的感染率约为30%。在总共 128 名受试者中，9.4% 和 18.0% 分别表现出中度（卵形细胞：30-49%）和重度（卵形细胞：50-100%）卵形细胞增多症。总共确定了三架 B3 Delta 27 航空公司。对数据集进行统计分析，我们观察到 (1) 较高的卵细胞率导致较低的疟疾感染，(2) B3 Delta 27 不能预防疟疾感染。这些结果表明，SAO 对疟疾的抵抗力是由于 SAO 没有 B3 Delta 27。
Genetic adaptation to malaria is a longstanding research topic in anthropology and human genetics. Southeast Asian ovalocytosis (SAO) has been documented to have resistance to malaria; however, the implications of existing data are controversial. In particular, SAO resistance to malaria is unlcear in terms of the types of SAO, with/without the 27-base pair deletion in the band 3 gene (B3 Delta 27). To shed light on the relationships between SAO and malaria, we surveyed Plasmodium infection, erythrocyte morphology, and the presence of the B3 Delta 27 among the residents of a selected area in East Kalimantan, Indonesia where malaria is endemic. We screened peripheral blood smears (n = 128) for Plasmodium infection and erythrocyte morphology under a microscope, and then DNA was extracted from the smears to be used as a template for a polymerase chain reaction to detect the B3 Delta 27. The prevalence of infection with Plasmodium including Plasmodium vivax and Plasmodium falciparum was approximately 30%. Among a total of 128 subjects, 9.4% and 18.0% showed moderate (ovalocytic cells: 30-49%) and severe (ovalocytic cells: 50-100%) ovalocytosis, respectively. A total of three B3 Delta 27 carriers were identified. The data set was statistically analyzed and we observed that (1) higher ovalocytic rate resulted in lower malaria infection and (2) the B3 Delta 27 did not prevent malaria infection. These results suggest that resistance of SAO to malaria is due to SAO without the B3 Delta 27.