Oral vaccination of mice against tetanus with recombinant Lactococcus lactis
Oral vaccination of mice against tetanus with recombinant Lactococcus lactis
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DOI：
10.1038/nbt0797-653
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发表时间：
1997-07-01
影响因子：
46.9
通讯作者：
LePage, RWF
中科院分区：
文献类型：
--
作者：
Robinson, K;Chamberlain, LM;LePage, RWF
为了确定是否可以通过口服递送重组细菌疫苗引起保护性免疫应答，破伤风毒素片段C（TTFC）在乳酸乳球菌中组成型表达并口服施用给C57 BL/6小鼠。所引发的抗体滴度低于鼻内免疫（已知导致高水平全身性抗TTFC免疫应答的途径）后的抗体滴度，但保护效力是相同的数量级。血清抗体同种型主要是IgG 1和IgG 2a。经口或鼻内免疫后可检测到TTFC特异性粪便伊加应答。通过鼻内途径给予化学灭活的乳球菌也能够引起与活细菌诱导的水平和动力学相似的血清抗体应答。
To determine whether a protective immune response could be elicited by oral delivery of a recombinant bacterial vaccine, tetanus toxin fragment C (TTFC) was expressed constitutively in Lactococcus lactis and administered orally to C57 BL/6 mice. The antibody titers elicited were lower than those following intranasal immunization (a route already known to result in high-level systemic anti-TTFC immune responses) but the protective efficacy was the same order of magnitude. The serum antibody isotypes elicited were predominantly IgG1 and IgG2a. TTFC-specific fecal IgA responses could be detected following oral Or intranasal immunization. Chemically killed lactococci administered via the intranasal route were also able to elicit serum antibody responses of similar levels and kinetics to those induced by live bacteria.