Preparation and suppressive effect of astragalus polysaccharide in glomerulonephritis rats
Preparation and suppressive effect of astragalus polysaccharide in glomerulonephritis rats
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DOI：
10.1016/j.intimp.2006.08.016
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发表时间：
2007-01-01
影响因子：
5.6
通讯作者：
Zhao, Jianxiong
中科院分区：
文献类型：
--
作者：
Li, Shigang;Zhang, Yongqi;Zhao, Jianxiong
黄芪（AM）在中医药中广泛用于治疗肾脏疾病。本研究从黄芪根茎中分离纯化出黄芪多糖（APS），其主要活性成分为D-吡喃葡萄糖，分子量为3.6 × 10（4）Da，以核转录因子-κ B（NF-κ B B）为指标，采用流式细胞术观察APS对阳离子牛血清白蛋白（C-BSA）诱导的大鼠肾小球肾炎的影响。通过ELISA方法测定白细胞介素-2（IL-2）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）。模型组和治疗组于第0天皮下注射C-BSA加不完全弗氏佐剂，第2 ~ 7周尾静脉注射C-BSA诱导肾小球肾炎。治疗组于第2 ~ 7周腹腔注射APS。治疗组NF-κ B B的表达及IL-2、IL-6、TNF-α的浓度明显降低。本研究清楚地表明APS通过抑制NF-κ B B介导的细胞因子途径对C-BSA诱导的肾小球肾炎具有保护作用。(c)2006 Elsevier B. V.保留所有权利。
Astragalus membranaceus (AM) has been widely used for treating kidney diseases in traditional Chinese medicine. In this study, the Astragalus polysaccharide (APS), the main active ingredient was isolated and purified from the Rhizomes of AM, which consisted Of D-glucopyranose and had the molecular weight of 3.6 x 10(4) Da. The effect of APS on glomerulonephritis rats induced by cationic Bovine Serum Albumin(C-BSA) was evaluated by flow cytometry using Nuclear Transcription Factor-kappa B (NF-kappa B) as marker. Interleukin-2 (IL-2), Interleukin-6 (IL-6) and Tumor Necrosis Factor-alpha (TNF-alpha) were determined by the ELISA method. The rats (model group and treatment group) were injected subcutaneously with C-BSA plus incomplete Freund's adjuvant on day 0, C-BSA was injected through the caudal vein from week 2 to week 7 to induce glomerulonephritis. The rats (treatment group) were given APS by intraperitoneal injection from week 2 to week 7. The expression of NF-kappa B and the concentration of IL-2, IL-6 and TNF-alpha were significantly decreased in the treatment group. This study clearly suggests that APS is effective in protecting against glomerulonephritis induced by C-BSA through the inhibition of NF-kappa B mediated-cytokine pathway. (c) 2006 Elsevier B.V. All rights reserved.