Antibodies to alcohol altered liver cell determinants in patients with alcoholic liver disease.
Antibodies to alcohol altered liver cell determinants in patients with alcoholic liver disease.
复制标题
酒精抗体改变了酒精性肝病患者的肝细胞决定因素。
作者：
J. Neuberger;I. Crossley;J. Saunders;M. Davis;B. Portmann;A. Eddleston;R. Williams
通过诱导细胞毒性和免疫荧光两种技术，证明了循环抗体与乙醇处理的兔肝细胞发生特异性反应。在细胞毒性试验中，19例酒精性脂肪肝患者中有7例(39%)、13例酒精性肝炎患者中有6例(46%)、36例肝硬变患者中有15例(43%)、14例肝炎和肝硬变患者中有7例(50%)出现抗体。在免疫荧光研究中，15份血清中有9份在乙醇处理的肝细胞上诱导了颗粒状荧光；两份诱导显著细胞毒性的血清没有诱导免疫荧光。在正常人或其他类型的急性或慢性肝病患者中未发现与酒精相关的抗体。这些结果表明，43%的酒精性肝病患者血清中存在针对乙醇改变的肝细胞决定簇的抗体，这表明慢性饮酒可能通过诱导肝细胞膜的抗原变化而触发细胞破坏性免疫反应。
Circulating antibodies reacting specifically with hepatocytes isolated from ethanol pretreated rabbits have been demonstrated by two techniques - induced cytotoxicity and immunofluorescence. In the cytotoxicity assay antibodies were found in seven of 19 (39%) of patients with alcoholic fatty liver (with or without fibrosis), six of 13 (46%) of those with alcoholic hepatitis, 15 of 36 (43%) of those with cirrhosis, and seven of 14 patients (50%) of those with hepatitis and cirrhosis. In the immunofluorescence studies, nine of 15 sera induced a granular pattern of fluorescence on the ethanol pretreated hepatocytes; two sera which induced significant cytotoxicity did not induce immunofluorescence. No ethanol related antibodies were found in normal individuals or in patients with other types of acute or chronic liver disease. These results show that antibodies directed against ethanol altered liver cell determinants are present in the serum of 43% of patients with alcoholic liver disease, and suggest a mechanism whereby chronic alcohol consumption may, by inducing antigenic changes in hepatocyte membranes, trigger a cell damaging immune reaction.