Isolation and biochemical characterization of partially transformation-defective mutants of avian myelocytomatosis virus strain MC29: localization of the mutation to the myc domain of the 110,000-dalton gag-myc polyprotein
Isolation and biochemical characterization of partially transformation-defective mutants of avian myelocytomatosis virus strain MC29: localization of the mutation to the myc domain of the 110,000-dalton gag-myc polyprotein
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禽骨髓细胞瘤病毒株 MC29 部分转化缺陷突变体的分离和生化特征：突变定位于 110,000 道尔顿 gag-myc 多蛋白的 myc 结构域
DOI：
10.1128/jvi.41.3.745-753.1982
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发表时间：
1982
影响因子：
5.4
通讯作者：
Michael J. Hayman
中科院分区：
文献类型：
--
作者：
Gary M. Ramsay;Michael J. Hayman
最近，我们分离了三种MC 29病毒突变体，它们虽然能够以与野生型MC 29相同的效率转化成纤维细胞，但转化巨噬细胞的效率低100倍。在这项研究中，我们发现，MC 29转化鹌鹑生产细胞系Q10能够产生这些部分转化缺陷突变体在高频率。使用胰蛋白酶肽图谱，我们确定了较小的gag-myc多聚蛋白编码的转化缺陷型病毒已经失去了myc特异性胰蛋白酶肽。这表明导致转化缺陷型病毒转化巨噬细胞效率低下的突变位于v-myc序列中，因此直接涉及MC 29转化中的v-myc和gag-myc多蛋白。
Recently, we isolated three mutants of MC29 virus which, although able to transform fibroblasts with the same efficiency as wild-type MC29, were 100-fold less efficient at transforming macrophages. In this study we found that MC29-transformed quail producer cell line Q10 was able to generate these partially transformation defective mutants at a high frequency. Using tryptic peptide mapping, we determined that the smaller gag-myc polyproteins encoded by the transformation-defective viruses had lost myc-specific tryptic peptides. This suggested that the mutations which resulted in the transformation-defective viruses being inefficient at transforming macrophages were located in the v-myc sequence and thus directly implicated v-myc and the gag-myc polyprotein in transformation by MC29.
禽急性白血病病毒的遗传结构。
DOI：
10.1101/sqb.1980.044.01.086
发表时间：
1980
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Bister,K;Duesberg,PH
通讯作者：
Duesberg,PH