Immunoglobulins reactive to carcinoembryonic antigen and their relationship to the antigen in malignant ascitic fluid of ovarian carcinoma
Immunoglobulins reactive to carcinoembryonic antigen and their relationship to the antigen in malignant ascitic fluid of ovarian carcinoma
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卵巢癌恶性腹水中癌胚抗原反应性免疫球蛋白及其与抗原的关系
DOI：
10.1002/ijc.2910300509
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发表时间：
1982
影响因子：
6.4
通讯作者：
E. Mackay
中科院分区：
文献类型：
--
作者：
R. Hill;S. Khoo;B. Daunter;P. Silburn;E. Mackay
检测了30例晚期卵巢癌患者的恶性腹水样本中是否存在CEA IgM抗体和结合125 I-CEA的PEG-可沉淀蛋白。使用卵巢癌胚抗原（CEA）通过固相放射免疫测定法测定抗CEA IgM抗体。CEA IgM抗体水平与体液中总IgM水平无相关性。在30例受试样本中的11例（37%）中，发现了显著量的CEA IgM抗体。CEA结合蛋白通过腹水将125 I-结肠-CEA掺入PEG-可沉淀复合物的能力来测量。在39个样品中的9个（30%）中，沉淀是显著的。ABO和刘易斯血型因子抗体与这些CEA抗体之间没有关联。两种测定法显示CEA水平与CEA结合蛋白水平之间呈反比关系。当125 I-CEA与这些“阳性”样品一起孵育时，通过色谱法证明了高分子量部分。相反，在“阴性”样本中，没有125 I-CEA掺入。这些发现表明CEA反应蛋白可能作为免疫复合物样物质存在于某些患者的腹水中。
Samples of malignant ascitic fluid from 30 patients with advanced ovarian carcinoma were examined for the presence of IgM antibodies to CEA and PEG‐precipitable proteins binding to 125I‐CEA. The IgM antibodies to CEA were measured by a solid‐phase radioimmunoassay using ovarian CEA. There was no correlation between the level of IgM antibodies to CEA and that of total IgM in the fluid. In 11 of 30 (37%) samples tested, significant amounts of IgM antibodies to CEA were found. The CEA‐binding proteins were measured by the ability of ascitic fluid to incorporate 125I‐colonic‐CEA into PEG‐precipitable complexes. In 9 of 39 (30%) samples, the precipitation was significant. There was no association between antibodies to the ABO and Lewis blood group factors and these antibodies to CEA. An inverse relationship was observed between the level of CEA and that of CEA‐binding proteins shown by the two assays. When 125I‐CEA was incubated with these “positive” samples, a high molecular weight fraction was demonstrated by chromatography. By contrast, in the “negative” samples, there was no incorporation of 125I‐CEA. These findings would indicate the presence of CEA‐reactive proteins possibly existing as immune‐complex‐like material in ascitic fluid of some patients.
使用癌胚抗原聚乙二醇免疫测定法证明癌症患者血清中抗刘易斯抗体升高。
影响因子：
11.2
作者：
Pompecki,R;Shively,JE;Todd,CW
通讯作者：
Todd,CW