Resolving Mechanisms of Competitive Fertilization Success in Drosophila melanogaster
Resolving Mechanisms of Competitive Fertilization Success in Drosophila melanogaster
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DOI：
10.1126/science.1187096
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发表时间：
2010-04-16
期刊：
影响因子：
56.9
通讯作者：
Pitnick, Scott
中科院分区：
文献类型：
--
作者：
Manier, Mollie K.;Belote, John M.;Pitnick, Scott
我们对交配后性选择的理解一直受到限制，因为无法区分不同雄性的竞争精子，再加上直接观察雌性生殖道内活精子的挑战。对由两只分别具有绿色和红色精子头的转基因雄性进行授精的雌性黑腹果蝇中精子运动、储存和使用的实时和时空分析使我们能够明确地区分精子优先的假设机制，包括第二个雄性对“常驻”精子的物理移位和丧失能力、雌性精子的射出以及精子的偏向使用。 精子竞争受精。我们发现，竞争性男性受精的成功源于涉及射精-女性和射精-射精相互作用以及复杂的精子体内行为的多变量过程。
Our understanding of postcopulatory sexual selection has been constrained by an inability to discriminate competing sperm of different males, coupled with challenges of directly observing live sperm inside the female reproductive tract. Real-time and spatiotemporal analyses of sperm movement, storage, and use within female Drosophila melanogaster inseminated by two transgenic males with, respectively, green and red sperm heads allowed us to unambiguously discriminate among hypothesized mechanisms underlying sperm precedence, including physical displacement and incapacitation of "resident" sperm by second males, female ejection of sperm, and biased use of competing sperm for fertilization. We find that competitive male fertilization success derives from a multivariate process involving ejaculate-female and ejaculate-ejaculate interactions, as well as complex sperm behavior in vivo.