Detection of St. Louis encephalitis virus antigen in mosquitoes by capture enzyme immunoassay
Detection of St. Louis encephalitis virus antigen in mosquitoes by capture enzyme immunoassay
复制标题
捕获酶免疫法检测蚊体内圣路易斯脑炎病毒抗原
DOI：
--
复制
发表时间：
1987
影响因子：
9.4
通讯作者：
J. Roehrig
中科院分区：
文献类型：
--
作者：
T. Tsai;R. A. Bolin;M. Montoya;R. Bailey;D. B. Francy;M. Jozan;J. Roehrig
监测方法，测量圣路易斯脑炎（SLE）病毒在自然界中的活动可能会提供预警，其流行病发生在人类。建立了一种抗原捕获酶联免疫分析法，用于检测感染蚊虫体内的SLE病毒。当抗原孵育过夜时，该测定检测到浓度为62 pg/0.1 ml的纯化SLE病毒抗原;在单日测定中检测到250 pg/0.1 ml（抗原孵育3 h）。该试验检测到67.9%和70.8%的实验室制备的池感染蚊子后3小时和过夜孵育，分别。该程序在鉴定感染性滴度大于dex 3.0的池时的灵敏度为90.5%。通过重新检测用SLE病毒和正常抗体预孵育的阳性合并液（导致含病毒抗原的合并液中的信号减弱）来控制试验的特异性。该程序在区分SLE和相关黄病毒方面具有适当的特异性，仅检测高感染剂量的异源抗原。
Surveillance methods that measure St. Louis encephalitis (SLE) virus activity in nature may provide forewarning of its epidemic occurrence in humans. An antigen capture enzyme immunoassay was developed to detect SLE virus in infected mosquitoes. The assay detected purified SLE viral antigen at a concentration of 62 pg/0.1 ml when antigen was incubated overnight; 250 pg/0.1 ml was detected in a single-day assay (antigen incubated for 3 h). The assay detected 67.9 and 70.8% of laboratory-prepared pools of infected mosquitoes after 3 h and overnight incubation, respectively. The sensitivity of the procedure was 90.5% in identifying pools with infectious titers greater than dex 3.0. The specificity of the assay was controlled by retesting positive pools preincubated with SLE virus and normal antibodies, which led to a diminution of signal in the pools containing viral antigen. The procedure was suitably specific in discriminating between SLE and related flaviviruses, detecting only high infectious doses of heterologous antigens.
通过酶免疫分析 (EIA) 检测蚊子池中的东部马脑脊髓炎病毒和高地 J 病毒抗原。
DOI：
10.4269/ajtmh.1984.33.965
发表时间：
1984
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Hildreth,SW;Beaty,BJ
通讯作者：
Beaty,BJ
通过酶免疫分析 (EIA) 检测蚊子池中的东部马脑脊髓炎病毒和高地 J 病毒抗原。
DOI：
10.4269/ajtmh.1984.33.973
发表时间：
1984
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Hildreth,SW;Beaty,BJ;Maxfield,HK;Gilfillan,RF;Rosenau,BJ
通讯作者：
Rosenau,BJ