Enteric adenoviruses: detection, replication, and significance
Enteric adenoviruses: detection, replication, and significance
复制标题
肠道腺病毒：检测、复制和意义
DOI：
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发表时间：
1979
影响因子：
9.4
通讯作者：
M. Petric
中科院分区：
文献类型：
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作者：
M. Retter;P. Middleton;J. Tam;M. Petric
通过电子显微镜或反免疫电泳可以很容易地在儿童胃肠炎患者的粪便中发现腺病毒，但在45%的病例中，病毒不能在细胞培养中生长。间接免疫荧光显微镜可用于检测腺病毒的非生长株；这些菌株具有独特的单细胞荧光模式。苏木精和伊红染色显示腺病毒样包涵体与荧光细胞分布相同。用恢复期患者血清预处理非生长腺病毒可中和其感染细胞培养物并产生荧光细胞的能力。间接免疫荧光显微镜与电子显微镜在显示患者粪便中的病毒方面比较有利。
Adenoviruses can be demonstrated readily in the stools of pediatric gastroenteritis patients by electron microscopy or counterimmunoelectrophoresis, but in 45% of these cases the virus will not grow in cell culture. Indirect immunofluorescence microscopy can be used to detect nongrowing strains of adenovirus; these strains have a unique single-cell fluorescence pattern. Hematoxylin and eosin staining reveals adenovirus-like inclusion bodies in the same distribution as fluorescent cells. Pretreatment of nongrowing adenovirus with convalescent-phase patient serum neutralized its ability to infect the cell culture and produce fluorescent cells. Indirect immunofluorescence microscopy compared favorably with electron microscopy in demonstrating virus in the stools of patients.