PCR-RFLP using Ssu-rDNA amplification: applicability for the diagnosis of mixed infections with different trypanosome species in cattle
PCR-RFLP using Ssu-rDNA amplification: applicability for the diagnosis of mixed infections with different trypanosome species in cattle
复制标题
DOI：
10.1016/j.vetpar.2003.08.004
复制
发表时间：
2003-11-14
影响因子：
2.6
通讯作者：
Geerts, S
中科院分区：
文献类型：
--
作者：
Delespaux, V;Ayral, F;Geerts, S
利用单限制性片段长度多态性(RFLP)-PCR方法对所有重要的牛锥虫物种进行了鉴定，以检测布氏锥虫、氏氏锥虫、刚果锥虫和间日锥虫的混合感染。结果表明，在浓度为10 ng ml（-1）的标准化DNA溶液中，混合感染的检出率至少为2%/98%。所有混合感染均具有清晰的特征，易于区分，但与刚果锥虫不同，刚果锥虫的特征带被掩盖。(C) 2003 Elsevier B.V.版权所有
The use of a single restriction fragment length polymorphism (RFLP)-PCR assay which is able to characterise all important bovine trypanosome species was evaluated for the detection of mixed infections with Trypanosoma brucei brucei, Trypanosoma theileri, Trypanosoma congolense and Trypanosoma vivax. Results showed that mixed infections are detectable at a minimum ratio of 2%/98% of standardised DNA solutions with a concentration of 10 ng ml(-1). All mixed infections gave clear profiles that could be easily differentiated except with T. theileri and T. congolense where the T. theileri band was concealed by the T. congolense profile. (C) 2003 Elsevier B.V. All rights reserved.