A SURVEY OF THE BINDING OF POLYCATIONIC FERRITIN IN SEVERAL FENESTRATED CAPILLARY BEDS - INDICATION OF HETEROGENEITY IN THE LUMINAL GLYCOCALYX OF FENESTRAL DIAPHRAGMS
A SURVEY OF THE BINDING OF POLYCATIONIC FERRITIN IN SEVERAL FENESTRATED CAPILLARY BEDS - INDICATION OF HETEROGENEITY IN THE LUMINAL GLYCOCALYX OF FENESTRAL DIAPHRAGMS
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DOI：
10.1016/0026-2862(83)90053-5
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
MILICI, AJ
中科院分区：
文献类型：
--
作者：
BANKSTON, PW;MILICI, AJ
将聚阳离子铁蛋白 (PCF) 注射到 17 天和 21 天妊娠大鼠的脐静脉以及年轻、雄性、成年 Sprague-Dawley 大鼠的隐静脉中，并使其循环 1 分钟或更短时间，然后切除组织并浸泡固定。检查胎儿肝脏、肠道和肾脏，以及成人肝脏、肠道、胰腺、肾脏、肾上腺皮质、骨髓和垂体。胎儿肠、成人肠、胰腺、垂体和肾管周毛细血管中几乎所有的窗孔隔膜，但没有内皮下结构，都被标记有一簇颗粒。甲状腺、肾上腺皮质和胎儿肾小球中覆盖窗孔的隔膜的一小部分具有相关的 PCF，但骨髓和胎儿肝脏中没有。在这些组织中，一些横膈膜似乎可渗透 PCF，因为在未标记的窗孔横膈膜下方紧邻基底层（胎儿肾脏、成人甲状腺和肾上腺皮质）或其他内皮下结构（胎儿肝脏、成人骨髓）中观察到簇绒。在成人肾小球基底膜的内层内层中还观察到 PCF 的周期性浓度。据报道，PCF 分别与成人肠和肾小球的窗膜和基底层结合，表明阴离子物质的积累。不仅证明了 PCF 与肠和胰腺以外的器官毛细血管中的窗膜的高特异性结合，而且还证明了其他器官中窗膜的 PCF 染色性的相当大的变化。
Polycationic ferritin (PCF) was injected into umbilical veins of 17- and 21-day-gestation rats and saphenous veins of young, male, adult Sprague-Dawley rats and allowed to circulate for 1 min or less followed by excision of tissues and immersion fixation. Fetal liver, intestine and kidney, as well as adult liver, intestine, pancreas, kidney, adrenal cortex, bone marrow and pituitary were examined. Nearly all fenestral diaphragms, but no subendothelial structures, were labeled with a tuft of particles in fetal intestine, adult intestine, pancreas, pituitary and renal peritubular capillaries. A fraction of the diaphragms covering fenestrae in the thyroid, adrenal cortex and fetal kidney glomerulus, but none in the bone marrow and fetal liver, had associated PCF. In these tissues some diaphragms appeared to be permeable to PCF, because tufts were observed immediately beneath unlabeled fenestral diaphragms either in the basal lamina (fetal kidney, adult thyroid and adrenal cortex) or on other subendothelial structures (fetal liver, adult bone marrow). Also periodic concentrations of PCF were observed in the lamina rara interna of the adult glomerular basement membrane. PCF binding to the fenestral diaphragms and the basal lamina in the adult intestine and the renal glomerulus, respectively, were reported as indicating accumulations of anionic material. Not only was high-specificity binding of PCF to fenestral diaphragms in capillaries of organs other than intestine and pancreas demonstrated, but also considerable variation of PCF stainability of fenestral diaphragms in other organs.